CELLine-two-compartment bioreactors for efficient protein expression



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CELLine

Disposable Bioreactor for Efficient Protein Expression

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Having troubles expressing enough recombinant protein?

Boost your production of
monoclonal antibodies or
recombinant proteins by
cultivating cells at highest
densities with CELLine, the
disposable bioreactor based on
Two-Compartment Technology.

Efficient protein expression
50-100 times higher product
concentrations compared to
classic cell culture disposables

Easy operation
as simple as using a tissue
culture flask

Cost efficient
90% less media supplements
and reduced handling time

Applications

Monoclonal antibody production in hybridomas
Recombinant protein expression in transfected cells
Virus production
Continuous culture maintenance for long-term studies
High-density cell culture

Easy and economical protein expression

Easy operation

Due to its uncomplicated design working with the CELLine bioreactor is as simple as with any standard tissue culture flask. For a straightforward control of the growth process, microscopic observation of the cells is made possible by the transparent design of the CELLine classic bioreactor. The system operates independently of any complicated control technology and works without any pump systems or agitation devices. Easily and securely stackable, CELLine flasks occupy a minimum of space in any standard CO2 incubator.
A specific adaptation of the cell culture techniques or media composition is generally not necessary when starting to work with CELLine and both, serum-supplemented or serum free media are suitable.

Cost efficiency

CELLine has been designed to bring substantial cost savings to cell cultivation. Labour costs are considerably reduced, because fewer disposable flasks need to be handled compared to other culture techniques in order to produce milligram amounts of protein. Also the expenses for media supplements are significantly reduced, since the addition of serum or other synthetic additives can be limited to the cell compartment. Furthermore, the high production yield and quality obtained in the relatively small culture volume of CELLine contribute to reduce costs and labour time in the subsequent downstream processing steps of the product.

Economic analysis for mAb Production

Units

CELLine 1000

T-Flask 225cm2

Productivity

Hybridoma productivity (literature value)

pg/h x cell

0.3

Cell density

cell/ml

3x10^7

1x10^6

Culture volume

Yield per harvest (7 days)

30.24

2.52

mAb concentration

mg/ml

1.51

0.05

Production Costs (250 mg mAb)

Amount

Cost (in $)

Amount

Cost (in $)

Harvests per disposable

Number of disposables

150

100

300

Medium ($ 20 per liter)

litres

160

100

Serum consumption* ($ 300 per liter)

4.8

500

150

Labour** ($ 25 per hour)

min

120

500

Total costs

364.8

758

Costs per mg mAb

1.46

3.03

*	The medium is supplemented with 10% of serum. In the case of CELLine, only the medium in the cell compartment is supplemented.
**	Labour is calculated as the time used for inoculating and harvesting one CELLine flask (15 min) or one T-Flask (5 min) multiplied by the number of harvests or flasks, respectively.

Animal welfare

CELLine is a disposable bioreactor that is competitive in costs and performance to the production of monoclonal antibodies using mice ascites. As an added benefit, when expressing monoclonal antibodies in hybridomas using CELLine, the antibody preparation is free of any contamination from mouse immunoglobulins. Over recent years, CELLine technology has been successfully adopted worldwide for the production of monoclonal antibodies and thereby has contributed diminishing the use of laboratory mice.

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