Efficient protein expression
Cells growing under the optimal conditions created in CELLine reach densities of 10^7 to 10^8 cells per ml, a cell concentration that is about two magnitudes higher than the one obtained with conventional culture techniques. Consequently, the concentration of expressed protein is typically 50 to 100 times above what is found in standard cell culture disposables. In addition, CELLine has been designed to maintain cells for several months in culture allowing to periodically harvest expressed proteins. By combining high product concentration with recurring product collection, large amounts of highly concentrated proteins are routinely obtained in CELLine. With classic culture techniques, contaminating proteins originating from serum or cells are a significant part of the total protein fraction. In contrast, due to the high product concentrations obtained with CELLine, the relative level of contaminating proteins to the expressed protein is much lower. In many cases the quality of monoclonal antibodies produced with CELLine is sufficient to perform standard laboratory tests, like Western Blot, without the need of any further purification steps. |
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Further Reading:
Efficient laboratory-scale production of monoclonal antibodies using membrane-based high-density cell culture technology. Trebak et al. (1999)
J. Immunol. Methods, 230: 59-70
Long-Term High Level Protein
Expression in Adherent, Protein-free Growing BHK Cells Using INTEGRA
CELLine adhere 1000 Bioreactor Flasks. J. Mittermaier and M. O. Zang- Gandor (2004) Genetic
Engineering News, 24(12): 42
For more references contact us at cell@integrabiosciences.
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The choice between CELLine classic and CELLine adhere makes it possible to grow both suspension or anchorage-dependent cells, hence allowing production of different biomolecules using different expression systems such as monoclonal antibodies in hybridomas, recombinant proteins in transfected cell lines and virus particles in packaging cells. |
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In vivo-type cultivation
Successful cultivation of different cell types demands not only skillful handling, but also depends to a large extent on the chosen cultivation system. CELLine technology closely mimics the physiological conditions within the body enabling production of drastically increased cell densities and more organotypic cell morphologies, sometimes even to the extent of 3-dimensional cell growth. |
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Representative EM micrographs showing the flat morphology of HEp-2 cells growing as monolayer
in a standard T-Flask (left) compared to the rounded shape of the same cells when cultivated
in CELLine adhere (right).
(With courtesy of W. Pfaller, Institute of Physiology, University of Innsbruck)
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