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CELLine - Technical Specification  
Bioreactor Types
CELLine classic (CL) is ideal for laboratory scale applications using suspension cells or adherent cells in combination with microcarriers. The unit is optimised for cultivation of hybridomas and many other cell types (e.g. CHO, NSO, SF cells).
     
CELLine adhere (AD) is specifically adapted to allow growth of anchorage-dependent cells (e.g. HEK, BHK, CHO cells). The bioreactor contains a woven, polyethylene terephtalate (PET) matrix in the cell compartment providing an ideal surface for cell attachement.
 
Technical Specifications
 
CELLine Two-Compartment Disposable Bioreactors are manufactured from optically clear virgin polysterene with a gas transfer bottom made of a molded silicone membrane providing a 0.2 µm vent barrier. The compartments are separated by a 10 kDa semi-permeable cellulose acetate membrane and individually pressure tested for integrity. The bioreactors are easily stackable owing to specific stabilisation interlocks, packed individually in easy to open medical-grade blister packaging, sterilised by gamma irradiation and non-pyrogenic.
 
  CELLine CL 350 CELLine CL 1000 CELLine AD 1000
Size: L x W x H (mm) 190 x 95 x 62 275 x 180 x 80 275 x 180 x 80
Weight (g) 185 334 336
Medium compartment cap 28 mm vented (0.2 µm), green polypropylene cap with polypropylene liner 38 mm vented (0.2 µm), white polypropylene cap with polypropylene liner 38 mm vented (0.2 µm), black polypropylene cap with polypropylene liner
Cell compartment cap 24 mm polypropylene cap with polyethylene liner 28 mm polypropylene cap with polyethylene liner 28 mm polypropylene cap with polyethylene liner
Cell compartiment inlay none none PET matrix inlay
Microscopic viewing
(inverted microscope) 		center window requires objective working distances of 2.5 mm center window requires objective working distances of 2.5 mm limited visibility due to inlay matrix
Vertical and horizontal
volume markings		 50 - 350 ml 100 - 1000 ml 100 - 1000 ml
 

 
 
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