| General Publications |
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| High density Cell Culture
in a new passive membrane based bioreactor |
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M. Wolf, T. DeSutter; Biotechnology
International 1999 |
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Celline
Overview, Application Introduction
This report is intended to be an overview of the CELLine
regarding its operation and the principle of cell compartment
viable cell capacity. This report describes the impact
of nutritional medium exchange and the diffusion of
solute accross the semi permeable membrane. The importance
of innoculation density and its impact on culture initiation
is described. The variable volume of the cell compartment,
expected osmotic water flux, and equivalency between
the different CELLine models is also reported. >more |
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M. Wolf; Wilson Wolf
Corporation,
Minneapolis USA |
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Small-scale Biomanufacturing Benefits from Disposable Bioreactors
In this article different disposable cell culture systems are compared and a cost analysis for production of MAbs using CELLine or conventional methods is presented.
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Fabrizio Baumann;
Biopharm International 2005, 18 (12): 22-30 |
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| Antibody Expression
in Hybridoma |
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Dialysis-based bioreactor
systems for the production of mono-clonal antibodies
- alternatives to ascites production in mice
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M.P. Bruce, V. Boyd, C. Duch, J.R. White;
Journal of Immunological Methods 2002, 264: 59-68 |
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Manufacture
of Pure Monoclonal Antibodies by Heterogeneous Culture
without Downstream Purification |
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L.E. Scott, H. Aggett,
D.K. Glencross; Biotechniques 2001, 31-3: 666-68 |
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Efficient
laboratory-scale production of monoclonal antibodies
using membrane-based highdensity cell culture technology
>more |
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M. Trebak, J.M. Chong, D. Herlyn, D.W.
Speicher; Journal of Immunological
Methods 1999, 230: 59-70 |
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Comparison of batch vs. Celline
culture for production of mono-clonal antibody in
vitro as alternatives to ascites. Application: Murine
Hybridoma
This report is a comparison of antibody production
in the CELLine compared to a traditional batch culture
method. The seven hybridoma clones which were cultured
in the CELLine had been previously cultured using
a traditional batch method. Comparison of results
obtained in the CELLine and in batch culture is provided.
The benefits of a concentrated product, reduced handling,
and reduced serum use are demonstrated. The consumption
of nutrient medium by the two different methods is
reported and provided for each clone. >more
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M. Wolf; Wilson Wolf
Corporation,
Minneapolis USA |
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Antibody
Manufacture in the Celline CL1000, Application: Murine
hybridoma
This report includes results from the
culture of over 30 different hybridoma clones in the
CELLine. The results from the large number of different
clones provides a range of performance which the user
can use to compare their individual results. Despite
significant differences which arise from clone to clone,
mean target values for cell numbers, antibody yield
and harvest times and volume are provided derived from
cultures of different hybridoma clones. Importantly,
the clones were randomnly selected representing numerous
mAb isotypes and fusion partners. Additionally, all
the cultures were carried out in a small manufacturing
environment aimed towards reducing overall costs and
labor. >more |
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M. Wolf; Wilson Wolf Corporation,
Minneapolis USA |
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Attachment
dependent cell cultivation on Micro-Carriers: Secre-ted
protein production >more
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M. Wolf; Wilson Wolf
Corporation,
Minneapolis USA |
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Membrane-based Cell cutlure systems - an
alternative to in Vivo production of monoclonal antibodies
A new generation of membrane based cell culture devices
especially designed for small scale production of
monoclonal antibodies (mab´s) entered the market
in last few years. In contrast to conventional perfusion
hollow fibre bioreactors these devices contain two
functionally different membranes - one ultrafiltration
membrane for nutrient supply and one gas permeable
membrane for direct oxygenation of cells. The latest
systems of this generation are static culture systems
are of moderate costs and either better than, or equal
to, the ascites mice in terms of quality and quantity
of produced monoclonal antibodies. We have investigated
the advantages of the perfused Tecnomouse bioreactor
and the static CELLine culture flasks in comparison
to ascites production and conventional roller bottle
cultures. >more
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A. Nagel, S. Koch, U. Valley,
F. Emmrich, U. Marx; Dev Biol Stand 1999, 101: 57-64 |
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Rapid
evaluation of hybridoma behaviour in high cell density
pro-duction process |
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S. Koch, A. Kriszo, C.
Kloth, U. Marx, A. Nagel; ESACT 1999, Lugano 1999 |
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Membrane-based
cell culture technologies: a scientifically and conomically
satisfactory alternative to malignant ascites produc-tion
for monoclonal antibodies |
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U. Marx; Res Immunol 1998, 149: 557-9 |
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| Protein expression
in CHO cells |
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| A soluble TGF-b type-1
Receptor mimics TGFb responses |
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F. Docagne, N. Colloc'h, V. Bougueret,
M. Page, J. Paput, M. Tripier, P. Dutartre,
E.T. MacKenzie, A. Buisson, S. Komesli, D. Vivien; Journal
of Biological Chemistry
2001, 276-49: 46243-50 |
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High
density suspension culture for Recombinant Protein produc-tion
from CHO cells >more |
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M. Wolf; Wilson Wolf Corporation,
Minneapolis USA |
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| Protein expression
in BHK cells |
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Long-Term
High Level Protein Expression in Adherent, Protein-free
Growing BHK Cells Using INTEGRA CELLine adhere 1000 Bio-reactor Flasks |
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J. Mittermaier, M. O. Zang-Gandor; EUGENEX
Biotechnologies GmbH,
Tägerwilen, Switzerland |
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| Production in Baculovirus
Infected SF9 Cells |
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Continuous Recombinant Protein Production in Baculovirus
Infected SF9 Cells using CELLine classic 1000 Two-Compartment Bioreactors |
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Izumi Matsumoto (GSI Creos GmbH, Tokio, Japan) and Alex Studer (INTEGRA Biosciences, Chur, Switzerland) |
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| Fermentation |
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The
Application of in vitro Models for Production of Metabolites:
Isolation and Characterisation of Hydroxysecobarbital |
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D. Marshall, M. Robinson, P. Hincks, M.
Dumasia, P. Teale, E. Houghton; hromatographia
Supplement 2000, 52: 35-38 |
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| Protein production in plant cells |
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Production of Human alpha-1-Antitrypsin from Transgenic Rice Cell Culture in a Membrane Bioreactor |
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McDonald KA et al. 2005,
21: 728-34 |
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