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TECNOMOUSE - Scientific Reports
 

1. Overviews, summaries and technology
2. Application protocols, references and scientific publications


1. Overviews, summaries and technology                 
 
Title Journal Language Authors Issue
Application of a hollow fiber membrane cell culture system in medicine Am Biotechnol Lab English U. Marx, H. Matthes,
A. Nagel, R.V. Baehr
1993, 11 (12): 26
Hollow fibre bioreactors: an alternative to murine ascites for small scale (<1 gram) monoclonal antibody production Res Immunol English N.L. Lipman, L.R. Jackson 1998, 149:571-6
Capitalising on capillaries Laboratory Technology International English U. Marx  
Monoclonal antibody production Atla English U. Marx, M.J. Embleton, R. Fischer, F.P. Gruber, et al. 1997, 25: 121-137
Engineering is speeding scale up and process development: Biotech's new mandate: more, cheaper, and faster   English    
Small scale monoclonal antibody production in vitro: methods and resources Lab Animal English L.R. Jackson, L.J. Trudel, N.S. Lipman 1999, 28: 38-50
Culture d'hybridomes sur Tecnomouse   French   Chapitre D
Production d'anticorps monoclonaux en fibre creuse à partir de différents hybridomes de souris   French J. Nedelc, J.-C. Laurent Chapitre E
Anticorps monoclonaux humains   French J.-J. Pin  
Culture d'hybridomes murins par la technique Tecnomouse: Suivi des paramètres biologiques et de la production d'immunoglobulines   French C. Daniel, A. Necker, G. Szekers, P. Zicavo, J. Hirn Chapitre B
Production d'anticorps monoclonaux (AcM) contre les groupes sanguins A et B   French A. Giannuzzo, N. Denize, C. Dubuy, M. Joussemet, Ph. Bourin Chapitre C
Entwicklung und Produktion monoklonaler Antikörper Bioforum German D. Baron 1995, 18: 373-82
In vitro Produktion durch Alternativen Verlag Gesundheit German U. Marx, S. Koch, K. Zühlke, H. Tanzmann, R. von Baehr 1993: 125-39
Austestung eines neuartigen Hohlfaserbioreaktors für die Kultivierung von Säugerzellen TF Berlin German A. Nagel 1993
Cell culture Facts BIOforum German Interview  

 

2. Application protocols, references and scientific publications                 > back to top

Choose your cell type:

Hybridoma
h Hybridoma
CHO
HEK
BHK
Primary
Primary rat hepatocytes
Liver
Bone marrow
h JAR choriocarcinoma
Determine O2 transfer
PBLs B-Cells


Hybridoma

Product Lit. Type Title Journal Authors Issue
  Application notes Rapid evaluation of hybridoma behavoiour in high cell density production process   S. Koch, A. Kriszo, C. Kloth, U. Marx, A. Nagel 1999
  Application notes Application protocols - Hybridomas: Monoclonal antibody protocols   U. Marx  
  Application notes Application protocols - Hybridomas: Cultivation of Hybridoma cells (Mouse IGG1 anti-Malaria) in the Tecnomouse   T. Jowett, A. O'Shea  
  Application notes Tecnomouse Technology: Cultivation of mouse hybridomas and human primary tissue in Tecnomouse   R. Biermann, A. Nagel, U. Marx, W. Merz  
IL-2 Application notes  Use of hollow-fiber bioreactor for production in problematic cell ines Genetic Engineering News O. Tzlanabos, R. Smith 1995, 143
IL-2 Application notes   Use of hollow-fiber bioreactor for production in problematic cell ines UK Product Review O. Tzlanabos, R. Smith 1995, June
MAb Application notes   Tecnomouse: Evaluation for monoclonal antibody production   T. Drew 1994
MAb Application notes   Recommendations for medium usage for Tecnomouse     1993
MAb Application notes   Removing difficult cells   R. Grimwood 1996
MAb Application notes   Current Methods for In vitro production of monoclonal antibodies   R. Grimwood  
MAb Scientific publication Development of optimized transfectoma cell lines for production of Chimeric antibodies in hollow fiber cell culture systems Biotechnology and Bioengineering B.S. Schläpfer, M. Scheibler, A.P. Holtorf, H. Van Nguyen, G. Pluschke 1995, 45: 310-319
MAb Scientific publication A Monoclonal antiidiotypic antibody ACA 125 mimicking the tumor-associated antigen CA 125 for immunotherapy of ovarian cancer Hybridoma H. Schlebusch, U. Wagner, U. Grünn, B. Schultes 1995, 14-2: 167-174
MAbs Scientific publication In vivo and in vitro Production of monoclonal antibodies: Bioreactors versus Immune Ascites Methods in Molecular Biology U. Marx, W. Merz Vol. 45
MAbs Scientific publication Evaluation of hollow fiber bioreactors as an alternative to murine ascites production for small scale monoclonal antibody production J Immunol Methods L.R. Jackson, L.J. Trudel, J.G. Fox, N.S. Lipman 1996, 189-2: 217-31
MAbs Scientific publication Analysis of a long term hybridoma culture in a new minimized high cell density bioreactor   S. Koch, H. Tanzmann, Ch. Riese, R. von Baehr, U. Marx 1992: 605-609
MAbs Scientific publication Rhoptry-associated protein 1-binding monoclonal antibody raised against a heterologous peptide sequence inhibits Plasmodium falciparum growth in vitro Infection and Immunity R. Moreno, F. Pöltl-Frank, D. Stüber, H. Matile, M. Mutz, N.A. Weiss, G. Pluschke 2001, 69: 2558-68
MAbs Scientific publication Epitope mapping of immunogenic and adhesive structures in repetitive domains of Mycoplasma bovis variable surface lipoproteins Infection and Immunity K. Sachse, J.H. Helbig, I. Lysnyansky, C. Grajetzki, W. Müller, E. Jacobs, D. Yogev 2000, 68: 680-87
MAbs Scientific publication Regional distribution and developmental changes of GluR1-Flop protein revealed by monoclonal antibody in rat brain J. Neurochem. J. Tönnes, B. Stierli, C. Cerletti, J.T. Behrmann, E. Molnar, P. Streit 1999, 73: 2195-205
MAbs Scientific publication Expression of the Neurofibromatosis Type 2 Gene in human tissues J. Histochem. & Cytochem. M.A. den Bakker, K.J. Vissers, A.C. Molijn, J.M. Kros, E.C. Zwarthoff, T.H. van der Kwast 1999, 47.11: 1471-1479
IgA, IgG Scientific publication Role of immunoglobulin A in proection against reovirus entry into Murine Peyer's Patches Journal of Virology K.J. Silvey, A.B. Hutchings, M. Vaijy, M.M. Petzke, M.R. Neutra 2001, 75-2: 10870-10879
IgA Scientific publication Production of immunoglobulin A in different reactor configurations Cytotechnology T. Stoll, C. Perregaux, U. von Stockar, I.W. Marison 1995, 17: 53-63
IgG Scientific publication Influence of bcl-2 on antibody productivity in high cell density perfusion cultures of hybridoma Cytotechnology D. Fassnacht, S. Rössing, R.P. Singh, M. Al-Rubeai, R. Pörtner 99, 30: 95-105
IgG Scientific publication Production of characterisation of monoclonal antibodies against a very small hapten, 3-methylindole J. Immunol. Methods M. Tuomola, R. Harpio, H. Mikola, P. Knuuttila, M. Lindström, V.-M. Mukkala, M.-T. Matikainen, T. Lövgren 2000, 240: 111-124
IgG Scientific publication Endothelial Tie receptor antigen in maternal and cord blood of healthy and preeclamptic subjects Obstetrics & Gynecology P. Vuorela, M-T. Matikainen, P. Kuusela, O. Ylikorkala, K. Alitalo, E. Halmesmäki 1998, 92: 179-183
IgG Scientific publication Circulating donor-specific cytotoxic T lymphocytes with high avidity for donor human leukocyte antigens in pediatric and adult cardiac allograft valved conduit recipients Europ. J. Cardio-thoracic Surgery F.B.S. Oei, M.J.P. Welters, C.J. Knoop, L.M.B. Vaessen, A.P.A. Stegmann, W. Weimar, A.J.J.C. Bogers 2000, 18: 466-72
IgG1 Scientific publication Fc-dependent and Fc-independent Opsonization of Cryptococcus neoformans by anticapsular monoclonal antibodies: Importance of epitope specificity Infection and Immunity D. Netski, T.R. Kozel 2002, 70: 2812-19
IgM Scientific publication Generation and characterization of a human monoclonal IgM antibody that recognizes a conserved epitope shared by lipopolysaccharides of different gram-negative bacteria Hybridoma M. Seifert, G. Schoenherr, D. Roggenbuck, U. Marx, R. von Baehr 1996, 15: 191-98
Abs Scientific publication Depletion of bovine CD8+ T cells with chCC63, a chimaeric mouse-bovine antibody Vet. Immunol. and Immunopath. C.J. Bruce, C.J. Howard, L.H. Thomas, P.R. Tempest, G. Taylor 1999, 71: 215-231
Abs Scientific publication VEGFR-3 and ist ligand VEGF-C are associated with angiogenesis in breast cancer Am. J. Pathol. R. Valtola, P. Salven, P. Heikkilä, J. Taipale, H. Joensuu, M. Rehn, T. Pihlajaniemi, H. Weich, R. deWaal, K. Alitalo  1999, 154: 1381-90
Abs Scientific publication Human IgG monoclonal antibodies that modulate the binding of specific IgE to birch pollen Bet v1 J. of Immunol. V. Visco, C. Dolecek, S. Denépoux, J. Le Mao, C. Guret, et al. 1996: 956-62
           
h Hybridoma   

Product Lit. Type Title Journal Authors Issue
IgM Scientific publication Effectiveness of vitamin A acetate for enhancing the production of lung cancer specific monoclonal antibodies Cytotechnology Y. Inoue, M. Fujisawa, Seiji Kawamoto, M. Shoji, S. Hashizume, M. Fujii, Y. Katakura, S. Shirahata 1999, 31: 77-83
           
CHO  

Product Lit. Type Title Journal Authors Issue
  Application notes   Application protocols - CHO Cells: CHO Cell Protocol   R. Grimwood 1995
  Application notes   Application protocols - CHO-Cells: Cultivation of CHO cells in the Tecnomouse-System   W. Moreth, K. Thomae  
Ab Scientific publication Construction of a bispecific single chain antibody for recruitment of cytotoxic T cells to the tumour stroma associated antigen fibroblast activation protein J. of Biotechnology T. Wüest, D. Moosmayer, K. Pfizenmaier 2001, 92: 159-68
           
HEK  

Product Lit. Type Title Journal Authors Issue
  Application notes   Application protocols - Spleen Cells: HEK 292 cell protocol      
r-apo Scientific publication   A novel kringle-4 number-based recombinant apo(a) standard for human apo(a) phenotyping Journal of Lipid Research E. Angles-Cano, S. Loyau, G. Cardoso-Saldana, R. Couderc, P. Gillery 1999, 40: 354-359
           
BHK, BHK21*   

Product Lit. Type Title Journal Authors Issue
 r-Epo Scientific publication  Efficient Production of Recobinant human erythropoietin by replenishment of microcarriers in the holow fiber culture cassette Biosci. Biotechnol. Biochem. Y. Inoue, N. Arita, K. Teruya, Y. Katakura, S. Shirahata 1999, 63: 1624-1626
Mab* Scientific publication  Production of a recombinant human monclonal antibody using a novel hollow fiber bioreactor system J. Fermentation and Bioeng. Y. Inoue, S. Kawamoto, K. Seki, K. Teruya, K. Mochizuki, M. Kato, et al. 1996, 81-5: 466-469
IgG Scientific publication  Production of recombinant human monoclonal antibody using ras-amplified BHK-21 cells in a protein-free medium Biosci. Biotechnol. Biochem. Y. Inoue, L. Bouzo Lopez, S. Kawamoto, N. Arita, K. Teruya, K. Seki, M. Shoji, M. Kamei, S. Hashizume, et al. 1996, 60: 811-817
           
Primary cells

Product Lit. Type Title Journal Authors Issue
  Application notes   Human Cancer and primary cell
culture in the new hybrid bioreactor
system tecnomouse
  A. Nagel, E. Effenberger, S. Koch,
L. Lübbe, U. Marx
 
  Application notes   Gewebekultivierung im Hohlfaserbioreaktor Biotec A. Nagel No.1, 1994
           
Primary rat hepatocytes

Product Lit. Type Title Journal Authors Issue
Metabolism Scientific publication   Culture of primary rat hepatocytes within a flat hollow fibre cassette for potential use as a component of a bioartificial liver suport system Biotechnology Letters K. Bratch, M. Al-Rubeai 2001, 23: 137-141
           
Liver cells

Product Lit. Type Title Journal Authors Issue
  Application notes   Of models & mimicry MLW J. McBride, J. Pugh, S. Oehlschlager, B. Hanley 1995 June
  Application notes   Kultivierung von immobilisierten Hepatozyten   Koebe  
           
Bone marrow, h thymic tissue*

Product Lit. Type Title Journal Authors Issue
  Application notes   Application Protocols - Bone Marrow protocols      
  Application notes   Application Protocols - Bone Marrow protocols: successfully growing bone marrow cells Laboratory News R. Biermann, R. Grimwood, U. Marx, W. Merz, T. DeSutter 1996
  Application notes   Dem ol' bones: Hollow fibre technology, which uses direct oxygenation, is allowing the successful culture of bone marrow cells Biotech. News R. Biermann, R. Grimwood, W. Merz, T. DeSutter 1996: ;March
  Abstract Establishment of organotypic bone marrow cultures of B-chronic lymphatic leukaemia   S.O. Hahn, W. Döcke, C. Liebenthal, H. Maerz, H. Matthes, H.D. Volk, F. Emmerich, U. Marx 1996
IL-6, IL-8* Abstract Human primary lymphoid tissue culture in the new hybrid bioreactor system Tecnomouse Abstract 24th meeting of the Society of Immunology A. Nagel, H. Matthes, U. Marx, R. von Baehr R.10, 237
           
h JAR choriocarcinoma

Product Lit. Type Title Journal Authors Issue
Xylosyl-
Transferase
Scientific publication   First isolation of human UDP-D-Xylose: Proteoglycan core protein b-D-Xylosyl-transferase secreted from cultured JAR choriocarinoma cells J. Biol. Chem. J. Kuhn, Ch. Götting, M. Schnölzer, T. Kempf, Th. Brinkmann, K. Kleesiek 2001, 276.7: 4940-4947
           
Determine O2 transfer

Product Lit. Type Title Journal Authors Issue
Physiology Scientific publication   Characterization of oxygen transfer in a membrane-aerated hollow-fibre bioreactor using modified microcoaxial needle electrodes Appl. Microbiol. Biotechnol. R. Wiesmann, S.T. Maier, U. Marx, R. Buchholz 1994, 41: 531-536
           
PBLs, B-Cells

Product Lit. Type Title Journal Authors Issue
Cells Scientific publication   Threedimensional cultures of B-Chronic lymphatic leukaemia cells derived from human bone marrow and peripheral blood in a miniaturized hollow fibre bioreactor Kluwer Academic S.O. Hahn, M. Wächter, H. Matthes, H.D. Volk, U. Marx 1995: 987-991

 
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