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VIAFLO 96 / 384 Electronic 96 and 384 Channel Pipette

   
   
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Customer feedback from SLAS 2013
Scientist review for VIAFLO 384
 
 
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VIAFLO 96 and VIAFLO 384 – Microplate pipetting made easy and affordable

Have you reached the limits of productivity with traditional hand held pipettes? Would you like to increase your pipetting throughput and reproducibility without investing in big automation?

VIAFLO 96 and VIAFLO 384 enable transfers of 96 and 384 samples in a single step, thus increasing pipetting throughput and reproducibility significantly. At a fraction of the cost, they often surpass throughput capabilities of even expensive robots.

 

Viaflo 96 handheld 96 channel pipette
VIAFLO 96 is an electronic, handheld 96 channel pipette designed to fit on any lab bench. The unique operating concept makes it as easy to use as any traditional handheld pipette.
Viaflo 384 handheld 96 channel pipette
VIAFLO 384 is an enhanced version of the successful VIAFLO 96. It offers the same intuitive operation and compact design, but allows working with both 96 and 384 channel heads for an even higher productivity.
 

Pipetting heads   (also click on tabs below for further information)

 

Always work in the optimal volume range

Choosing the appropriate pipetting head for the application’s volume range helps to achieve the best pipetting results in terms of accuracy and precision. The heads are available in five volume ranges: 0.5 – 12.5 µl, 2 - 50 µl, 5 – 125 µl, 10 – 300 µl and 50 – 1250 µl. Changing a pipetting head is easy and takes less than a minute.

VIAFLO 384 can operate with 96- and 384-channel heads while VIAFLO 96 works with 96-channel heads only.

All channels of a pipetting head have their own pistons, making every channel independent from each other to avoid cross-channel influences. It is also possible to load the pipetting head only partially with tips. For example load only 8 tips on one column to perform serial dilutions.


Viaflo 96/384 Head Change

GripTips for reliable pipetting results

 

Loosing a tip during an assay preparation can ruin the entire assay and a reliable sealing is of key importance for accurate pipetting.

INTEGRA has redesigned the connection between pipette tip and tip fitting to resolve this issue. The unique GripTip pipette tips match perfectly with the Tri-Lobe tip fitting to form an integral system. During loading the tips snap over three lobes, which mechanically holds them on the tip fitting. It effectively prevents tips from falling off and guarantees a perfect sealing on every tip to prevent sample spillage.
Visit the GripTip product page to learn more about the innovative GripTip system.

The GripTips are comfortably loaded and ejected without any physical effort due to a power assisted mechanism activated by a push button. The loading procedure also guarantees that GripTips are not touched by the user and remain sterile.

Viaflo 96/384 Tip Loading

Space saving footprint

 

VIAFLO 96 and VIAFLO 384 are compact and use little bench space.

They can also be easily relocated and fit into most laminar flow cabinets for sterile applications.

Up to three plate holders accommodate tip racks, microplates, reagent reservoirs or other SBS-size labware.

Up to 8 microplates can be stacked to make maximum use of the stage positions.

  Viaflo 96/384 Benchtop Pipette

Plate holder overview

 

VIAFLO 96 and VIAFLO 384 feature two deck positions
(A and B) to accommodate different plate holders.

They can be removed and replaced by another option
any time to adapt the instrument to various application requirements. 

 

  VIAFLO 96 and VIAFLO 384 feature two deck positions
   
Standard plate holder   Spring loaded plate holder with slide function
     
Standard plate holder
Plate holder with slide function

For 96 channel pipetting. The standard plate holder (6205) is used for simple 96 well transfers. The plate holder with slide function (6210) enables a grid offset to access 384-well plates with a 96-channel head.
  Spring loaded plate holder with slide function - 384 well plate offset
For 96 and 384 channel pipetting.
Available for position A (6215) and B (6220), these plate holders feature a spring load mechanism to secure microplates in place. Recommended for applications that require an exact plate positioning. Both holders also feature the slide function to access 384 well plates with a 96 channel head.
     
Three position stage  
     

Three position stage
For 96 and 384 channel pipetting.
The stage provides three plate holder positions. Move the stage to the left and right to work with all three positions. It features the spring mechanism and slide function on all three positions.
The three position stage occupies both positions A and B and cannot be combined with another plate holder.

 

Adjustable spring loaded plate holders with slide function – 384 well plate offset
For 96 and 384 channel pipetting.
Available for position A (6223) and B (6224) these plate holders feature a slide function to access 384 well plates with a 96 channel head. A spring load mechanism secures the microplate in place. Both holders are adjustable in y direction to allow an optimal alignment of the pipetting head with the target plate. Recommended for 384 well plates with small wells, such as round well plates.

     
  Row dilution plate holder
     

Adjustable spring loaded plate holders with slide function – 1536 well plate offset
For 96- and 384 channel pipetting.
Available for position A (6225) and B (6226) these plate holders feature a slide function to pipet into 1536 well plates with a 384-channel pipetting head. A spring load mechanism secures the microplate in place. Both holders are adjustable in y direction to allow an optimal alignment of the pipetting head with the target 1536 well plate.

  Row dilution plate holder
In addition to serial dilutions in columns, this plate holder enables serial dilutions in  rows. It works for both 96 and 384 well plates.
Plate holder inserts: PCR cooling plate    
     

Plate holder inserts: PCR cooling plate
These aluminum 96 and 384 well PCR cooling plates keep reagents cooled during sample preparation in a PCR plate.
The cooling plates feature an SBS footprint, fit into any of our plate holders but can also be used for other applications or with other instruments.

   

A comprehensive electronic pipette

 

VIAFLO 96, VIAFLO 384 and all other VIAFLO electronic pipettes have a common user interface making it easy enough to switch between both systems without a second thought.

A full color screen and proven touch wheel interface offer quick and comprehensible menu navigation. Simply by moving the finger over the touch wheel, menus and settings can be accessed and changed.

The multilingual userinterface features full text menus in English, German, French, Chinese and Japanese.

Viaflo User Interface

Full range of pipetting modes
A choice of ten predefined pipetting modes provides users with convenient solutions for the most common liquid handling tasks. Standard pipetting routines, such as plate to plate transfers or multiple dispenses from a reservoir to different plates, can be executed with minimal set up time.

VIALINK for convenient pipette and program management
VIAFLO 96 and VIAFLO 384 can be connected to a computer via USB port. With the free to use pipette management software VIALINK, custom programs can be created on the computer and then be transferred onto VIAFLO 96/384. For laboratories with many users or power users with many pipetting protocols, all custom programs can be stored and backed up in a library on the computer. For further information visit our VIALINK Pipette Management software page.

Reservoir to plate transfers   (also click on tabs below for further information)

  • Compound additions
  • Medium exchange
  • Cell seeding
  • Addition of buffers and reagents (e.g. ELISA handling)

VIAFLO 96/384 fills microplates within seconds by transferring up to 384 samples simultaneously. It reduces pipetting errors and increases pipetting reproducibility as all samples are transferred the same way.

Time to fill five 96-well microplates

Time to fill five 96-well microplates [seconds]

Reservoir to plate transfers

The “Repeat dispense” function is an extremely effective mode to fill multiple plates with the same liquid: Aspirate once and dispense multiple aliquots of the same or different volume. Plates can be stacked during the process to minimize plate handling.

Plate to plate transfers

  • Plate duplication
  • Supernatant transfers
  • Plate reformatting 96 well <-> 384 well

 

Plate to plate transfers

An exact aspiration height can be defined to precisely remove supernatant. The ability to adjust the pipetting speed helps to gently aspirate without disturbing cells at the bottom of a well.

Transfers within plates

  • Serial Dilutions
  • Mixing and Resuspension

Performing serial dilutions, mixing or resuspending contents of entire plates can effortlessly accomplished without repeatedly pressing a plunger. The number of mixing cycles and the mixing speed can be exactly defined.

Transfers within plates

Nucleotide and protein extraction in 96-well format

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In this video we demonstrate how the vacuum manifold AURUM from Biorad can be used with VIAFLO 384 to extract RNA. The manifold fits directly on the plate holders of VIAFLO 384 without modifications and can be used right away.
 
Windows Media Video (WMV)
(runs on Windows PC)
MP4 format, based on Quick Time
(runs on iPhones, iPads)
       
VIAFLO384_Vacuum_Manifold
(WMV, 720x1280, 105 MB)
VIAFLO384_Vacuum_Manifold
(MP4, 720x1280, 122 MB)

1536-well pipetting

1536-well pipetting
 

The video explains how the special 1536-well plate holder enables easy and fast 1536-well pipetting with VIAFLO 384.

 
Windows Media Video (WMV)
(runs on Windows PC)
MP4 format, based on Quick Time
(runs on iPhones, iPads)
       
1536-well-Plate-Holder_V00
(WMV, 720x1280, 24 MB)
1536-well-Plate-Holder_V00
(MP4, 720x1280, 60 MB)
VIAFLO 96 / 384 Electronic 96 and 384 Channel Pipette
 
Windows Media Video (WMV)
(runs on Windows PC)
MP4 format, based on Quick Time
(runs on iPhones, iPads)
       
VIAFLO 96 (WMV, 720x1280, 53 MB) VIAFLO 96 (MP4, 720x1280, 100 MB)
    VIAFLO 96 (MP4, 480x270, 27 MB)

Pipetting Head Overview

Part number 6101 / 6131 6106 / 6136 6102 / 6132 6103 6104
Volume range [μl] 0.5 – 12.5 2 – 50 5 – 125 10 – 300 50 – 1250
Channels 96 / 384 96 / 384 96 / 384 96 96
VIAFLO 96 6101 6106 6102
VIAFLO 384 6101 / 6131 6106 / 6166 6102 / 6132

Specifications

Part number 6101 / 6131 6106 / 6136 6102 / 6132 6103 6104

Test volume (µl)

1.25 6.25 12.5
5 25.0 50
12.5 62.5 125
30 150 300
125 625 1250
Accuracy [± %]
8.0 2.0 1.5
5.0 2.0 1.5
3.0 2.0 1.5
2.5 1.5 1.2
2.5 2.0 1.8
Precision [ %]
3.0 1.5 1.0
2.5 1.5 1.0
1.7 1.5 1.0
1.5 1.0 0.8
1.5 1.0 0.8
 
Technical data VIAFLO 96 VIAFLO 384
Channels 96 96, 384
Pipetting technology air displacement
Compatible plate formats  96- and 384-well microplates, shallow and deep well
Dimensions (W x D x H)  42 cm x 30 cm x 54 cm (17" x 12" x 21")
Weight 25.7 kg (56.7 lbs) 27.7 kg (61.1 lbs)
Operating conditions  Temperature: 5 °C – 40 °C, Humidity: <85 % non-condensing

Power requirements

 100 – 240 VAC, 50/60 Hz

Instruments   (also click on tabs below for further information)

Click on image for a larger view   Part No.

VIAFLO 96
96-channel handheld pipette
Viaflo 96 - 96-channel handheld pipette

(Click on image for a larger view)

Base unit
Plate holders and pipetting heads not included
6001
VIAFLO 384
96- and 384-channel handheld pipette
Viaflo 384 - 96- and 384-channel handheld pipette

Base unit
Plate holders and pipetting heads not included


6031

 

Plate holders

Add one plate holder for each stage position on A and B
For a detailed description of the plate holder refer to the Plate Holder Overview in the Features tab.

    Part No.

Row Dilution Plate HOlder for row-wise serial dilutions

Standard Plate Holder – For pipetting in 96-well plates

6205

Row Dilution Plate HOlder for row-wise serial dilutions

Plate Holder with slide function – For plate reformatting
96 well <-> 384 well

6210
Row Dilution Plate HOlder for row-wise serial dilutions

Spring Loaded Plate Holder A with slide function – Recommended for 384-well pipetting applications and plate reformatting

6215
Row Dilution Plate HOlder for row-wise serial dilutions

Spring Loaded Plate Holder B with slide function – Recommended for 384-well pipetting applications and plate reformatting

6220
Row Dilution Plate HOlder for row-wise serial dilutions

Row Dilution Plate Holder – Enables serial dilutions in rows of 96- and 384-well plates

6221
Row Dilution Plate for row-wise serial dilutions

Three Position Stage (occupies both deck positions A and B) – Suitable for 96- and 384-well applications

6230

Adjustable spring loaded plate holder A with slide function (384 well plate offset)

6223

Adjustable spring loaded plate holder B with slide function (384 well plate offset)

6224

Adjustable spring loaded plate holder A with slide function (1536 well plate offset)

6225

Adjustable spring loaded plate holder B with slide function (1536 well plate offset)

6226

Pipetting heads

    Part No.
96-channel Pipetting Heads
96-channel Pipetting Heads

0.5 – 12.5 μl 6101
2 – 50 μl 6106
5 – 125 μl 6102
10 – 300 μl 6103
50 – 1250 μl 6104
   
384-channel Pipetting Heads
384-channel Pipetting Heads

0.5 – 12.5 μl 6131
2 – 50 μl 6136
5 – 125 μl 6132
 
Note: 50 μl pipetting heads are only compatible with instruments featuring a 2nd generation control unit
(Firmware 3.xx or higher).
 

Replacement O-rings

300 μl O-ring, tip fitting, replacement kit, 24/pack
100-00027-50
300 μl O-ring, tip fitting, replacement kit, 100/pack 125 928
1250 μl O-ring, tip fitting, replacement kit, 24/pack 100-00028-50
1250 μl O-ring, tip fitting, replacement kit, 100/pack 125 929

GripTips for use with VIAFLO 96 and VIAFLO 384

    Part No.
12.5 μl GripTips
XYZ 12.5 ul GripTips

5 XYZ racks of 384 tips, non-sterile, LONG 6403
5 XYZ racks of 384 tips, sterile, LONG 6404
5 XYZ racks of 384 tips, sterile, filter, LONG 6405
5 XYZ racks of 384 tips, non-sterile 6453
5 XYZ racks of 384 tips, sterile 6454
5 XYZ racks of 384 tips, sterile, filter 6455
5 XYZ racks of 384 tips, filter, SHORT 6475
 
50 /125 μl GripTips
XYZ 125 ul GripTips
5 XYZ racks of 384 tips, non-sterile 6463
5 XYZ racks of 384 tips, sterile 6464
5 XYZ racks of 384 tips, sterile, filter 6465
 
300 μl GripTips
GripTip box for 300 microliters

5 inserts of 96 tips, non-sterile, GREEN CHOICE
6432
5 racks of 96 tips, non-sterile 6433
5 racks of 96 tips, sterile 6434
5 racks of 96 tips, sterile, filter 6435
 

1250 μl GripTips
1250 microliters

5 inserts of 96 tips, non-sterile, GREEN CHOICE
6442
5 racks of 96 tips, non-sterile 6443
5 racks of 96 tips, sterile 6444

5 racks of 96 tips, sterile, filter

 

 

6445

Reagent reservoirs for VIAFLO 96 and VIAFLO 384

    Part No.
Reservoir Base
Reservoir Base
SBS Standard Footprint (10 bases per case) 6300
Disposable Reagent Reservoirs
(incl. 2 Reservoir Bases)
Disposable Reagent Reservoirs
100 ml, 96 well, 30 reservoirs individually sealed, sterile 6311
100 ml, 96 well, 200 reservoirs per case, bulk pack, sterile 6312
Disposable Reagent Reservoirs
(incl. 2 Reservoir Bases)
Disposable Reagent Reservoirs
300 ml, 96 well, 30 reservoirs individually sealed, sterile 6321
300 ml, 96 well, 200 reservoirs per case, bulk pack, sterile 6322
Disposable Reagent Reservoirs
(incl. 2 Reservoir Bases)
384-well reagent reservoir
100 ml, 384 well, 30 reservoirs individually sealed, sterile 6331
100 ml, 384 well, 200 reservoirs per case, bulk pack, sterile 6332
300 ml 96 well Polypropylene
Reservoir
96 well polypropylene
25 reservoirs per case, pyramid bottom, non-sterile 6351
25 reservoirs per case, pyramid bottom, pre-sterilized 6352
12 column Polypropylene
Reservoir
12 column polypropylene
25 reservoirs per case, pyramid bottom, non-sterile
6361
25 reservoirs per case, pyramid bottom, pre-sterilized 6362
8 row Polypropylene Reservoir
25 reservoirs per case, pyramid bottom, non-sterile
6371
25 reservoirs per case, pyramid bottom, pre-sterilized 6372
 

Accessories

    Part No.
PCR 96-well Cooling Plate
PCR 96-well Cooling Plate
6250
PCR 384-well Cooling Plate
PCR 384-well Cooling Plate
6255
 
 

Flyer VIAFLO 96/384
Flyer

Operating Instructions  VIAFLO 96/384
Operating Instructions


Vialink
VIALINK
  Video

Windows Media Video (WMV)
(runs on Windows PC)
MP4 format, based on Quick Time
(runs on iPhones, iPads)
 
VIAFLO 96 (WMV, 720x1028, 53 MB)   VIAFLO 96 (MP4, 720x1028, 100 MB)
VIAFLO384_Vacuum_Manifold (WMV, 720x1028, 105 MB)   VIAFLO 96 (MP4, 480x270, 27 MB)
    VIAFLO384_Vacuum_Manifold (MP4, 720x1028, 122 MB)
     
 
Testimonials  
VIAFLO 96 to replicate collections of yeast strains and perform genetic screenings in 96 well plates.

Improved Efficiency of Replication & Screening

The Institute of Genetics and Development of Rennes (FR) uses the VIAFLO 96 to replicate collections of yeast strains and perform genetic screenings in 96 well plates.

> more

Molecular Partners from Zurich (CH) integrated a VIAFLO 96 in their protein expression workflow. The pipette is used for various steps in their IMAC protein purification protocol.

96-well High Throughput Protein Expression

Molecular Partners from Zurich (CH) integrated a VIAFLO 96 in their protein expression workflow. The pipette is used for various steps in their IMAC protein purification protocol.

> more

Customer feedback from SLAS 2013

Efficient Library Screening

PepsiCo Global R&D from White Plains NY (USA) uses a VIAFLO 384 to screen natural product libraries to find sugar substitutes. The 384-channel pipette is used to transfer compound plates to multiple cell plates.

Watch Video

Reducing Protocol Time for 96-well PCR Assays

A leading US clinical diagnostic laboratory has significantly improved the reproducibility and productivity of their Real-time PCR assays using a VIAFLO 96 handheld electronic pipette.
> more

 
   
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