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DOSE IT - Applications  

Efficient coating or washing of dishes or tissue culture flasks
Coating of dishes or tissue culture flasks is a common procedure in laboratories involved in cell culture. Using the peristaltic pump DOSE IT results in significant time-savings and at the same time helps to reduce excessive strain caused by this repetitive work. Read the Application Note for detailed information.

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Graph showing a customized mode for coating/washing 10 Petri dishes.    

Filling of density gradient tubes
Density gradient centrifugation is frequently used to separate different cell types or cell organelles, ribosomes or DNA from crude cellular extracts. The CUSTOMIZE mode permits gentle and fast building of density step gradients. To avoid stirring up the different layers, dispensing starts gently at a low flow rate and then ramps up to the target speed. This allows filling density gradient tubes gentle and faster than if carried out manually.
   
 
Graph showing a customized mode for filling 10 density gradient tubes.    

Serial dilutions
Serial dilutions are extensively used for example in microbiological testing as part of the sample preparation for the total viable cell count in food. By programming an air gap before taking up the sample into the prefilled tubing system, the CUSTOMIZE mode allows you to efficiently carry out serial dilutions.
   
     
 
Graph showing a customized mode for carrying out serial dilutions.    
 
 
 
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