MAGFLO™ NGS Magnetic beads for NGS size selection
Magnetic beads for NGS size selection
Seamless integration, superior results:
MAGFLO NGS for library prep
MAGFLO NGS magnetic beads provide a reliable solution for the purification of nucleic acid fragments with a consistent size distribution, which is crucial for NGS library preparation workflows. This includes both single- and double-sided size selection methods for DNA or RNA, as they are free of RNases.
An advantage of using magnetic beads for NGS size selection is that they allow protocol automation, increasing the reproducibility of results. To automate MAGFLO NGS magnetic bead workflows, we recommend combining the beads with our range of benchtop liquid handling instruments and MAG or HEATMAG modules to provide streamlined, hands-free purification and size selection workflows. This provides an end-to-end solution from a single trusted supplier.
How it works
MAGFLO NGS magnetic beads are used for size selection in NGS library preparation, ensuring consistent DNA or RNA fragment sizes.
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Overview
- MAGFLO NGS magnetic beads are RNase free, making them suitable for both DNA and RNA workflows.
- Precise bead-to-sample ratios ensure easy target size selection, offering effective targeting of nucleic acid fragments in both single- and double-sided size selection protocols without needing to change bead ratios when using the gold standard beads.
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MAGFLO NGS sets the new gold standard
MAGFLO NGS beads are interchangeable with gold standard magnetic beads for size selection protocols. This makes MAGFLO NGS magnetic beads a cost-effective alternative for almost any lab. View the application note comparing MAGFLO NGS beads and gold standard beads to see full data.
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Proven performance in sequencing workflows
MAGFLO NGS and gold standard magnetic beads were compared in a complex amplicon sequencing workflow, and generated reproducible results. See the data poster.
RNA sequencing (RNA-Seq) workflows with MAGFLO NGS provide reliable NGS results, highlighting their comparable efficacy and reliability with gold standard magnetic beads for generating high quality data. See the data poster.
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Integration with MAG and HEATMAG modules
- MAGFLO NGS magnetic beads are seamlessly complemented by the MAG and HEATMAG modules – dynamic magnetic stands designed for efficient bead handling – ensuring reliable and consistent sample processing.
- These modules use vertical magnet movements to automate bead collection, eliminating the need for manual plate transfers to reduce the risk of spills.
- MAG and HEATMAG modules are compatible with various tube and plate formats to meet diverse research needs.
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Integration with our pipetting platforms
- MAGFLO NGS magnetic beads and MAG and HEATMAG modules can be combined with our advanced pipetting platforms – such as the ASSIST PLUS pipetting robot, or VIAFLO 96 or VIAFLO 384 handheld electronic pipettes – to enable semi- or fully automated workflows, eliminating manual errors and optimizing productivity and reproducibility.
- The ASSIST PLUS is ideal for workflows with variable sample numbers. This versatile platform offers completely hands-free automation and user-friendly programming.
- The VIAFLO 96 and VIAFLO 384 can handle 96 or 384 samples simultaneously and offer semi-automated pipetting, making them ideal for high throughput applications.
- Our all-in-one solutions provide optimized pipetting protocols which can be easily modified in just a few clicks to accommodate differing input and output volumes, bead-to-sample ratios and target fragment sizes.
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Le produit en action
Utilisations et applications
Applications
- NGS library clean-up
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NGS library clean-up
NGS size selection during library preparation
Magnetic beads are adept at selectively isolating DNA fragments according to their size, a critical step for both single- and double-size selection in NGS library preparation. Employing MAGFLO NGS beads with MAG or HEATMAG modules, combined with the precision offered by INTEGRA's pipetting platforms, for magnetic bead purification ensures high yields while minimizing bead loss.
- Single size selection
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Single size selection
Left or right size selection of nucleic acid fragments
MAGFLO NGS magnetic beads feature a single magnetic bead-to-sample ratio in single size selection.
- Double size selection
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Double size selection
Left and right size selection of nucleic acid fragments
MAGFLO NGS magnetic beads feature two specific ratios in double-sided size selection.
- DNA amplicon sequencing
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DNA amplicon sequencing
Library preparation for the Illumina MiSeq™ System
Amplicons of 16S rRNA and internal transcribed spacer (ITS) were obtained from a microbial community standard containing genomic DNA from 8 bacterial species (3 Gram-negative and 5 Gram-positive) and 2 yeasts. This study shows the comparable performance of INTEGRA and AMPure XP magnetic beads when used in size selection for NGS library preparation.
- RNA-Seq
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RNA-Seq
Comparative analysis of gene expression patterns in RNA sequencing
RNA-Seq is an indispensable tool in modern biology, using NGS to analyze the complexity of gene expression dynamics and molecular pathways across diverse experimental conditions. In this comprehensive analysis, we show the interchangeability of INTEGRA MAGFLO NGS and AMPure XP magnetic beads in nucleic acid clean-ups for RNA-Seq of human reference RNA samples.
Spécifications
Veuillez trouver ici un bref aperçu des spécifications du produit. Des informations plus détaillées sont disponibles dans la section téléchargement ci-dessous.
- Application
- Size selection for NGS library prep
- Starting material
- Fragmented DNA, RNA, cDNA, PCR products
- Elution volume
- 12 µl or above (standard 96 well PCR plate)
- Fragment size
- 100-1000 bp, depending on bead-to-sample ratio used
- Typical recovery
- ≥85 % (1.8x single size)
- Processing mode
- Automated and manual
- Throughput
- 1-96 samples
- Downstream applications
- Library preparation for NGS, PCR, gDNA target size selection, fragment analysis, microarrays, enzymatic reactions, cloning
- Selling unit
- 1, 50 and 500 ml
- Storage
- 2-8 °C
- Intended use
- Research use only
- RNase free
- yes
Téléchargements & Plus
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Flyer - MAGFLO™ Magnetic beads for nucleic acid purification and extraction ( 2.35 Mo ) -
Safety Data Sheet - MAGFLO NGS - DE ( 400.89 Ko ) -
Safety Data Sheet - MAGFLO NGS - EN ( 430.2 Ko ) -
Poster - RNA sequencing - MAGFLO NGS vs. AMPure XP (PDF) ( 1.81 Mo ) -
Poster - Amplicon sequencing - MAGFLO NGS vs. AMPure XP (PDF) ( 1.18 Mo ) -
Manuel d’instructions MAGFLO NGS ( 780.5 Ko )
Références articles
- Magnetic beads
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Magnetic beads
MAGFLO NGS
Description Part No. MAGFLO NGS, magnetic beads for NGS size selection, 1 ml, tube 7000 MAGFLO NGS, magnetic beads for NGS size selection, 50 ml, bottle 7002 MAGFLO NGS, magnetic beads for NGS size selection, 500 ml, bottle 7004 - Magnetic separation modules
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Magnetic separation modules
MAG and HEATMAG modules and accessories
Description Part No. MAG module for magnetic bead separation 4900 HEATMAG module for heating and magnetic bead separation 4901 Adapter for 1.5 ml tubes, 4 x 6 format (MAG/HEATMAG) 4905 Adapter for 96 well PCR plate (MAG/HEATMAG) 4906 Adapter and magnetic array for 384 well PCR plate (MAG) 4908 Adapter and magnetic array for deep well PCR plate (MAG) 4907 - ASSIST PLUS and D-ONE
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ASSIST PLUS and D-ONE
Please visit these webpages for the ASSIST PLUS pipetting robot and the D-ONE single channel pipetting module.
- 96 channel electronic pipettes
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96 channel electronic pipettes
Please visit these webpages for the MINI 96 and the VIAFLO 96 and VIAFLO 384 electronic pipettes.
- VIAFLO and VOYAGER electronic pipettes
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VIAFLO and VOYAGER electronic pipettes
Please visit these webpages for the VIAFLO lightweight electronic pipettes and the VOYAGER adjustable tip spacing pipettes.