MAGFLO™ PCR Magnetic beads for PCR purification
Magnetic beads for PCR purification
Precision purification for PCR:
Unlock the potential with MAGFLO PCR
MAGFLO PCR magnetic beads streamline nucleic acid purification for PCR clean-up to provide samples that are ready for direct use in downstream applications, such as mutation detection and genotyping, sequencing, fragment analysis, microarrays, enzymatic reactions and cloning. With a simple, 3-step protocol, MAGFLO PCR magnetic beads rapidly and efficiently remove unincorporated dNTPs, excess primers, primer dimers, salts and enzymes to deliver a high amplicon recovery rate.
A significant advantage of using MAGFLO PCR magnetic beads for PCR purification workflows is that they allow protocol automation, increasing the reproducibility of results. This can be carried out quickly as centrifugation and filtration are not required. To automate MAGFLO PCR workflows, we recommend combining the beads with our range of benchtop liquid handling instruments and MAG and HEATMAG modules to provide streamlined, hands-free purification workflows with components from a single trusted supplier.
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Montrer toutHow it works
MAGFLO PCR magnetic beads are ideal for enhancing the efficiency of PCR reaction purification protocols, providing samples that are immediately ready for use in downstream applications, such as mutation detection and genotyping, sequencing, fragment analysis, microarrays, enzymatic reactions and cloning.
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Overview
- In a simple, 3-step protocol, MAGFLO PCR delivers a high recovery rate of amplicons larger than 100 bp.
- Centrifugation and filtration are not required, ensuring that protocols can be carried out quickly.
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Effortless PCR clean-up, enhanced efficiency
MAGFLO PCR magnetic beads rapidly and efficiently remove unincorporated dNTPs, excess primers, primer dimers, salts and enzymes, as shown for samples C1-H2 in the image on the left. View the application note with MAGFLO PCR beads to see full data.
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Integration with our MAG and HEATMAG modules
- MAGFLO PCR magnetic beads seamlessly complement the MAG and HEATMAG modules – dynamic magnetic stands designed for efficient bead handling – to ensure reliable and consistent sample processing.
- These modules have magnets that move vertically to automate bead collection, eliminating the need for manual plate transfers to reduce the risk of spills.
- The MAG and HEATMAG modules are compatible with tubes and various plates to support the scalability of MAGFLO PCR magnetic beads to meet diverse research needs, ensuring effortless reproducibility.
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Integration with our pipetting platforms
- MAGFLO PCR magnetic beads and MAG and HEATMAG modules can be combined with our advanced pipetting platforms – such as the ASSIST PLUS pipetting robot, or VIAFLO 96 or VIAFLO 384 handheld electronic pipettes – to enable semi- or fully automated workflows, eliminating manual errors and optimizing productivity and reproducibility.
- The ASSIST PLUS is ideal for workflows with variable sample numbers. This versatile platform offers completely hands-free automation and user-friendly programming.
- The VIAFLO 96 and VIAFLO 384 can handle 96 or 384 samples simultaneously, and offer semi-automated pipetting, making them ideal for high throughput applications.
- Our all-in-one solutions provide optimized pipetting protocols which can be easily modified in just a few clicks to accommodate differing input and output volumes, bead-to-sample ratios and target fragment sizes.
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Le produit en action
Utilisations et applications
Applications
- PCR purification
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PCR purification
Achieve high recovery rate of amplicons
It's crucial to clean up reaction mixes following PCR protocols to eliminate any unincorporated primers or dNTPs, preventing them from interfering with subsequent reactions. Combining MAGFLO PCR beads with a MAG module equipped with an adapter for 96 or 384 well PCR plates enables hands-free PCR clean-up for up to 384 reactions simultaneously.
Spécifications
Veuillez trouver ici un bref aperçu des spécifications du produit. Des informations plus détaillées sont disponibles dans la section téléchargement ci-dessous.
- Application
- PCR clean-up
- Starting material
- PCR products, fragmented DNA
- Elution volume
- 12 µl or above (standard 96 well PCR plate)
- Fragment size
- Min. 100 bp and larger
- Typical recovery
- Above 85 %
- Processing mode
- Automated or manual
- Throughput
- 1-384 samples
- Downstream applications
- PCR, mutation detection and genotyping, Sanger sequencing protocols, fragment analysis, microarrays, enzymatic reactions, cloning
- Selling unit
- 1, 50 and 500 ml
- Storage
- 2-8 °C
- Intended use
- Research use only
- RNase free
- No
Références articles
- Magnetic beads
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Magnetic beads
MAGFLO PCR
Description Part No. MAGFLO PCR magnetic beads for PCR purification, 1 ml, tube 7010 MAGFLO PCR magnetic beads for PCR purification, 50 ml, bottle 7012 MAGFLO PCR magnetic beads for PCR purification, 500 ml, bottle 7014 - Magnetic separation modules
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Magnetic separation modules
MAG and HEATMAG modules and accessories
Description Part No. MAG module for magnetic bead separation 4900 HEATMAG module for heating and magnetic bead separation 4901 Adapter for 1.5 ml tubes, 4 x 6 format (MAG/HEATMAG) 4905 Adapter for 96 well PCR plate (MAG/HEATMAG) 4906 Adapter and magnetic array for 384 well PCR plate (MAG) 4908 Adapter and magnetic array for deep well PCR plate (MAG) 4907 - ASSIST PLUS and D-ONE
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ASSIST PLUS and D-ONE
Please visit these webpages for the ASSIST PLUS pipetting robot and the D-ONE single channel pipetting module.
- 96 channel electronic pipettes
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96 channel electronic pipettes
Please visit these webpages for the MINI 96 and the VIAFLO 96 and VIAFLO 384 electronic pipettes.
- VIAFLO and VOYAGER electronic pipettes
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VIAFLO and VOYAGER electronic pipettes
Please visit these webpages for the VIAFLO lightweight electronic pipettes and the VOYAGER adjustable tip spacing pipettes.