MAGFLO PCR magnetic beads in 1ml, 50ml and 500ml bottles

MAGFLO™ PCR Magnetic beads for PCR purification

Magnetic beads for PCR purification

Product story

Precision purification for PCR: 
Unlock the potential with MAGFLO PCR

MAGFLO PCR magnetic beads streamline nucleic acid purification for PCR clean-up to provide samples that are ready for direct use in downstream applications, such as mutation detection and genotyping, sequencing, fragment analysis, microarrays, enzymatic reactions and cloning. With a simple, 3-step protocol, MAGFLO PCR magnetic beads rapidly and efficiently remove unincorporated dNTPs, excess primers, primer dimers, salts and enzymes to deliver a high amplicon recovery rate. 

A significant advantage of using MAGFLO PCR magnetic beads for PCR purification workflows is that they allow protocol automation, increasing the reproducibility of results. This can be carried out quickly as centrifugation and filtration are not required. To automate MAGFLO PCR workflows, we recommend combining the beads with our range of benchtop liquid handling instruments and MAG and HEATMAG modules to provide streamlined, hands-free purification workflows with components from a single trusted supplier.

How it Works

How it works

MAGFLO PCR magnetic beads are ideal for enhancing the efficiency of PCR reaction purification protocols, providing samples that are immediately ready for use in downstream applications, such as mutation detection and genotyping, sequencing, fragment analysis, microarrays, enzymatic reactions and cloning.

  • Overview

    Illustration of the PCR purification principle, with different sized amplicons and primer dimer removal.
    • In a simple, 3-step protocol, MAGFLO PCR delivers a high recovery rate of amplicons larger than 100 bp.
    • Centrifugation and filtration are not required, ensuring that protocols can be carried out quickly. 
  • Effortless PCR clean-up, enhanced efficiency

    MAGFLO PCR magnetic beads remove primers

    MAGFLO PCR magnetic beads rapidly and efficiently remove unincorporated dNTPs, excess primers, primer dimers, salts and enzymes, as shown for samples C1-H2 in the image on the left. View the application note with MAGFLO PCR beads to see full data.

  • Integration with our MAG and HEATMAG modules

    MAG and HEATMAG all compatible labware adapters
    • MAGFLO PCR magnetic beads seamlessly complement the MAG and HEATMAG modules – dynamic magnetic stands designed for efficient bead handling – to ensure reliable and consistent sample processing. 
    • These modules have magnets that move vertically to automate bead collection, eliminating the need for manual plate transfers to reduce the risk of spills.
    • The MAG and HEATMAG modules are compatible with tubes and various plates to support the scalability of MAGFLO PCR magnetic beads to meet diverse research needs, ensuring effortless reproducibility.
  • Integration with our pipetting platforms

    ASSIST PLUS and VIAFLO 96 size selection MAG
    • MAGFLO PCR magnetic beads and MAG and HEATMAG modules can be combined with our advanced pipetting platforms – such as the ASSIST PLUS pipetting robot, or VIAFLO 96 or VIAFLO 384 handheld electronic pipettes – to enable semi- or fully automated workflows, eliminating manual errors and optimizing productivity and reproducibility.
    • The ASSIST PLUS is ideal for workflows with variable sample numbers. This versatile platform offers completely hands-free automation and user-friendly programming.
    • The VIAFLO 96 and VIAFLO 384 can handle 96 or 384 samples simultaneously, and offer semi-automated pipetting, making them ideal for high throughput applications.
    • Our all-in-one solutions provide optimized pipetting protocols which can be easily modified in just a few clicks to accommodate differing input and output volumes, bead-to-sample ratios and target fragment sizes.

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See it Work

See it work

Apps

Uses and Applications

Overview Applications Stories

Applications

PCR purification
PCR purification

Achieve high recovery rate of amplicons

It's crucial to clean up reaction mixes following PCR protocols to eliminate any unincorporated primers or dNTPs, preventing them from interfering with subsequent reactions. Combining MAGFLO PCR beads with a MAG module equipped with an adapter for 96 or 384 well PCR plates enables hands-free PCR clean-up for up to 384 reactions simultaneously.

VIAFLO 384 transfers etoh to mag
Tech Info

Tech info

Find here a quick overview of the product specifications. More detailed information can be found in the download-section below.

Application
PCR clean-up
Starting material
PCR products, fragmented DNA
Elution volume
12 µl or above (standard 96 well PCR plate)
Fragment size
Min. 100 bp and larger
Typical recovery
Above 85 %
Processing mode
Automated or manual
Throughput
1-384 samples
Downstream applications
PCR, mutation detection and genotyping, Sanger sequencing protocols, fragment analysis, microarrays, enzymatic reactions, cloning
Selling unit
1, 50 and 500 ml
Storage
2-8 °C
Intended use
Research use only
RNase free
No
Downloads & More Parts and Numbers

Parts and numbers

Magnetic beads
Magnetic beads

MAGFLO PCR

DescriptionPart No.
MAGFLO PCR magnetic beads for PCR purification, 1 ml, tube7010
MAGFLO PCR magnetic beads for PCR purification, 50 ml, bottle7012
MAGFLO PCR magnetic beads for PCR purification, 500 ml, bottle7014
Magnetic separation modules
Magnetic separation modules

MAG and HEATMAG modules and accessories

DescriptionPart No.
MAG module for magnetic bead separation4900
HEATMAG module for heating and magnetic bead separation4901
Adapter for 1.5 ml tubes, 4 x 6 format (MAG/HEATMAG)4905
Adapter for 96 well PCR plate (MAG/HEATMAG)4906
Adapter and magnetic array for 384 well PCR plate (MAG)4908
Adapter and magnetic array for deep well PCR plate (MAG)4907
ASSIST PLUS and D-ONE
ASSIST PLUS and D-ONE

Please visit these webpages for the ASSIST PLUS pipetting robot and the D-ONE single channel pipetting module.

96 channel electronic pipettes
96 channel electronic pipettes

Please visit these webpages for the MINI 96 and the VIAFLO 96 and VIAFLO 384 electronic pipettes.

VIAFLO and VOYAGER electronic pipettes
VIAFLO and VOYAGER electronic pipettes