Observing good pipetting practice helps immensely to improve reproducibility and accuracy. Selection of good pipetting techniques, which if maintained offer the best chance of increasing and maintaining the reproducibility of your serial dilutions. Mixing is a key component of dilution protocols and in this article we additionally discuss different mixing parameters to help you achieve a homogenous sample. To maximize consistency of dilution assays, automating the process offers many benefits. For ensuring highly reproducible serial dilutions we outline what features an automated pipetting system should have and introduce the VIAFLO ASSIST automated pipette – a product purpose designed for this task.