MEDIACLAVE 10/30 Media Sterilizer
Fast, reproducible and safe media sterilization
The preparation of agar medium is a routine task in today’s laboratories. The typical workflow is to dissolve the components in a flask followed by sterilization in an autoclave. However, this procedure has several disadvantages.
First, the numerous working steps makes media preparation very time-consuming. Second, the sterilization temperature is usually controlled only in one single reference container, with the result that heat exposure may differ from sample to sample. Making things worse, the agar medium within the bottles cannot be stirred during the sterilization process – which otherwise would guarantee that it stays homogeneous. Lastly, it takes very long to cool the medium to dispensing temperatures and hence is exposed to a high temperature for a long time. As a result, the fertility of the agar medium might be impaired.
INTEGRA has designed a dedicated media sterilizer which eliminates these disadvantages. MEDIACLAVE allows for a much shorter process in which medium formulation and sterilization is carried out in one single step. The temperature of the agar medium is precisely monitored and controlled during the entire process. Permanent stirring guarantees homogenous temperature throughout the whole batch and an efficient plate heat exchanger facilitates rapid cooling. All these features prevent the medium from excessive heat exposure and thereby maintains its fertility.
How it works
The MEDIACLAVE product range allows the rapid and gentle preparation of 1 – 30 L culture medium. Precise controlling and monitoring of temperature, time and pressure during the sterilization process guarantee constant high quality.
MEDIACLAVE is equipped with several independent monitoring systems for pressure and temperature, guaranteeing highest safety standards for the user and the working environment. The vessel lid is equipped with an autonomous overpressure safety valve and a burst disc in case all other electronic monitoring systems fail.
1) Adding Port
2) Overpressure Safety Valve
3) Dispensing Port
4) Temperature Probe Pt1000
Fast Heating and Rapid Cooling
Powerful heating elements permit fast media processing. This minimises thermal stress and ensures high fertility of your culture medium. Pressure and temperature controlled deaeration guarantees saturated steam in the vessel.
Rapid cooling is enabled by an efficient plate heat exchanger. The separation of the cooling water system and the sterilization chamber makes it unlikely that culture media gets contaminated by cooling water.
1) Standard mode: Heating up, sterilization and cooling down to dispensing temperature.
2) Chocolate agar mode: After the first sterilization phase, blood is added and the medium heated up again.
The large and strong magnetic stirrer with adjustable speed and reversing rotation direction ensures homogenous media preparation over a wide viscosity range. A Pt-1000 temperature probe and microprocessor control of process parameters allow reproducible and complete sterilization of culture media.
MEDIACLAVE can be quickly and conveniently connected to the dispensing tubing of the automated Petri dish filler MEDIAJET. Alternatively, the peristaltic pump DOSE IT can be used to fill containers of more unusual volumes or shapes, e.g. quadrangular Petri dishes, bottles or flasks.
Large containers such as bottles can be filled by pressure dispensing. Medium is dispensed by automatically building up pressure inside the sterilization chamber.
Process documentation and validation
The MEDIACLAVE sterilization process is monitored and can be stored on a PC. A digital signature according to FDA (21 CFR Part 11) / EU (GMP Annex 11) is automatically added to the electronic process file. All logfiles can also be stored using the MEDIACLAVE USB port.
If equipped with an optionally available dot matrix printer, high quality printouts insusceptible to fading can be archived.
See it work
Uses and Applications
- Standard Agar medium
Standard Agar medium
MEDIACLAVE used for the preparation and sterilization of numerous types of culture media
Typical examples are:
- LB (Luria Bertani) Medium – primarily used for the cultivation of Escherichia coli
- Tryptic Soy Agar – general purpose medium used for culturing a wide array of microorganisms
- Blood Agar – for fastidious microorganisms
- Chocolate Agar – for fastidious respiratory bacteria such as Haemophilus influenzae
- MacConkey Agar – for Gram-negative bacteria
- Sabouraud Agar – for fungi
- Peptone solutions – for sample preparation
- Charcoal Agar
Preparing Charcoal Agar Medium using MEDIACLAVE
Charcoal Agar is used for the cultivation and isolation of Bordetella pertussis and Haemophilus influenza among others. Using a stirrer bar with paddles, high-viscosity Charcoal Agar can be prepared without the need of pre-swelling. Preparation time is reduced by more than 30 percent.
Benefits using MEDIACLAVE with paddle stirrer:
- Improved mixing performance
- Minimized foaming
- Shorter preparation time
- Fly food preparation
Fly food preparation
An optimized system for the preparation of Drosophila melanogaster (fly) food
MEDIACLAVE is an attractive alternative to simple cooking pots or stirrer kettles often used for preparing Drosophila (fly) food. With its integrated cooling system, MEDIACLAVE eliminates the need to wait several hours until the fly food is cooled to dispensing temperature. In no more than 90 minutes, the mixture is ready to dispense. In addition, the fly food is kept at the desired dispensing temperature
Find here a quick overview of the product specifications. More detailed information can be found in the download-section below.
- MC 10: 1-10 litres; MC 30: 3-30 litres
- Dimensions (HxWxD)
- MC 10: 480 x 550 x 640 mm; MC 30: 1040 x 550 x 640 mm
- MC 10: 57 kg; MC 30: 85 kg
Parts and numbers
MEDIACLAVE 10 and MEDIACLAVE 30
10 Litres Media Preparator complete with cuvette, lid seal, magnetic stirrer, decanting tubing, fitting for dispensing tubing, integrated printer and printer splash guard (200 – 240 V, 50/60 Hz).
Plug Part No. EU (CEE 7/7) 136 000 US (NEMA L6-30P) 136 010 No plug 136 020
MEDIACLAVE 10 (without printer)
10 Litres Media Preparator complete with cuvette, lid seal, magnetic stirrer, decanting tubing, fitting for dispensing tubing (200 – 240 V, 50/60 Hz).
Plug Part No. EU (CEE 7/7) 136 005 US (NEMA L6-30P) 136 015 No plug 136 025
30 Litres Media Preparator complete with cuvette, lid seal, magnetic stirrer, decanting tubing, fitting for dispensing tubing, integrated printer and printer splash guard.
Voltage Part No. 3 x 200 – 208 V, 50/60 Hz (US, JP) - with plug 136 050 3 x 400 V, 50/60 Hz (EU) - without plug 136 055
- Accessories for MEDIACLAVE 10
Accessories for MEDIACLAVE 10
Cuvettes, stirrer bars, adapters, etc.
Description Part No. Stainless steel cuvette for medium sterilization 136 030 Autoclave kit for autoclaving/thermostatting liquids in containers, incl. autoclave cuvette, stainless steel with grid insert and flexible temperature probe 136 070 Magnetic stirrer bar for homogeneous medium mixing within the cuvette 132 130 Magnetic stirrer bar with paddle for homogeneous stirring of high viscosity agar medium 136 075 Decanting tubing for insertion into the cuvette for dispensing, incl. Silicone tubing, stainless steel nozzle and securing nut 136 034 Fitting for dispensing tubing for connecting tubing (inner diameter 6 mm) to dispense port, incl. stainless dispense port fitting and spring 136 035 Printer splash guard for protecting the integrated printer from splashes 136 040 Tubing connector for adding port, for adding volumes of supplements through the adding port using a silicone tubing (inner diameter 6 mm) and a peristaltic pump 136 049 Injection lid for sterile injection of supplements though the adding port, incl. cap, punched disc and septum membrane (silicone/PTFE) 136 247 Pressure dispensing kit for direct pressure dispensing, incl. Pinch valve box, foot switch, silicone tubing and stainless steel dispensing tube 136 064 Dispensing tube for pressure dispensing, inner diameter 6 mm, length 10 cm, stainless steel, one end dented 171 056 Foot switch with connecting cable for pressure dispensing, to trigger the pinch valve 143 200
- Accessories for MEDIACLAVE 30
Accessories for MEDIACLAVE 30
Cuvettes, stirrer bars, adapters, etc.
Description Part No. Stainless steel cuvette for medium sterilization, incl. stainless steel guide tube for temperature probe 136 060 Magnetic stirrer bar with paddle for homogeneous stirring 136 075 Decanting tubing for insertion into the cuvette for dispensing, incl. rigid stainless steel tube (length 613 mm), silicone tubing and securing nut 136 061 Fitting for dispensing tubing for connecting tubing (inner diameter 6 mm) 136 035 Printer splash guard for protecting the integrated printer from splashes 136 040 Tubing connector for adding port, for adding volumes of supplements through the adding port using a silicone tubing (inner diameter 6 mm) and a peristaltic pump 136 049 Injection lid for sterile injection of supplements though the adding port, incl. cap, punched disc and septum membrane (silicone/PTFE) 136 247 Pressure dispensing kit for direct pressure dispensing, incl. Pinch valve box, foot switch, silicone tubing and stainless steel dispensing tube 136 064 Dispensing tube for pressure dispensing, inner diameter 6 mm, length 10 cm, stainless steel, one end dented 171 056 Foot switch with connecting cable for pressure dispensing, to trigger the pinch valve 143 200 Volume measuring stick for convenient measurement of the product volume in the cuvette 136 565
Consumables for MEDIACLAVE 10 and MEDIACLAVE 30
Description Part No. Lid seal for sealing the vessel lid, silicone 135 860 Pivot Pin Disk for cuvette 10/30 L, PTFE, 6-pack 136 066 Septum membrane for injection lid, silicone/PTFE, self-resealing, 10-pack 136 047 Paper rolls for integrated dot matrix printer, 10-pack 136 038 Ink ribbon for integrated dot matrix printer 136 901 Silicone tubing for pressure dispensing, length 25, (bulk roll), inner diameter 6 mm, autoclavable 171 036