How does magnetic bead-based DNA isolation work?

Magnetic bead-based isolation protocols operate through a series of pipetting steps for lysis, binding, washing and elution – using magnetic separation to isolate the nucleic acids of interest. Unlike traditional spin column-based purification methods, magnetic bead-based workflows do not rely on cumbersome centrifugation or vacuum manifolds to bind and elute nucleic acid, making them ideal for automated DNA isolation protocols.

The Clean Blood & Tissue DNA Kit workflow consists of four easy stages. First, the sample is treated with a lysis buffer to break open cells, release nucleic acids and denature proteins along with other impurities. Next, magnetic beads are added to the lysate, allowing gDNA to selectively bind to the bead surface in the presence of a binding buffer. This is followed by several wash steps designed to remove residual proteins, salts and other contaminants, ensuring high gDNA purity. In the final stage, the purified gDNA is released from the magnetic beads into an elution buffer, making it ready for downstream applications. Heating can be applied during both the lysis and elution phases to enhance DNA yields, offering users added flexibility based on their specific requirements.

Reliable performance across multiple sample types

The Clean Blood & Tissue DNA Kit is engineered to process a wide range of input materials – including blood, buffy coat, tissue and cultured cells – offering high versatility for a range of laboratories. Typical recoveries range from 5 to 7 µg of DNA, and the protocol supports both manual and automated processing of samples. This flexibility makes the kit ideal for variable throughput needs, from benchtop set-ups to fully automated workflows.

High purity and qPCR-ready DNA

The Clean Blood & Tissue DNA Kit delivers consistent DNA yields with notably high purity:

• OD A260/A230 values are close to the reference range of 2.0 to 2.2, indicating low levels of organic contaminants and salts.
• OD A260/A280 values are consistent with protein-free DNA preparations (~1.8).
• qPCR compatibility was proven by showing no inhibition of signal in the dilution series of the eluate.

This highlights the reliability and suitability of DNA extracted with the Clean Blood & Tissue DNA Kit for sensitive downstream applications.

Automated magnetic bead separation with HEATMAG modules

The Clean Blood & Tissue DNA Kit is fully compatible with our HEATMAG module, an advanced magnetic separation device designed for reliable, highly efficient magnetic bead handling in a range of labware formats, including 1.5 ml tubes, deep well plates, and 96 or 384 well PCR plates. This module uses automated vertical magnet movement to capture beads in one location, enabling precise aspiration to reduce sample loss and eliminating the need for manual transfers between stands to avoid the risk of cross-contamination. This is combined with precise temperature control to allow heating during lysis and elution steps for maximum DNA recovery. 

Seamless integration with INTEGRA pipetting platforms

The Clean Blood & Tissue DNA Kit can be used as a standalone product, but its real power comes as part of an all-in-one solution. Combined with the HEATMAG module and our advanced pipetting platforms – such as the ASSIST PLUS pipetting robot – it enables automated DNA extraction workflows, eliminating manual errors and optimizing productivity and reproducibility.

Designed for flexibility 

The Clean Blood & Tissue DNA Kit is available for 16, 96 or 384 extractions and comes with all necessary reagents, including proteinase K. We also offer magnetic beads for NGS size selection and PCR purification, providing labs with a one-stop shop for automated magnetic bead-based purification.

*The Clean Blood & Tissue DNA Kit is available in Austria, Belgium, Canada, Denmark, France, Germany, Ireland, Luxembourg, Sweden, Switzerland, the Netherlands, the United Kingdom, and the United States.