Stage 2: Library preparation
The challenge
Small manual pipetting errors in library prep can quickly compound, affecting bead ratios, cleanup efficiency, and ultimately library consistency. INTEGRA automates these critical repetitive steps, helping laboratories to reduce variability and support consistent execution during NGS library preparation.
Simple set-up for low to medium throughput
VOYAGER adjustable tip spacing pipette + ASSIST PLUS pipetting robot
Plate-to-plate transfers, reagent additions and mixing steps are common manual tasks in library preparation.
Pair the VOYAGER with ASSIST PLUS to take these steps off your hands. Choose your protocol, press start and run hands-free for consistent pipetting across samples.
Check out the VOYAGER and ASSIST PLUS
MIRO CANVAS NGS prep system
Ready to push automation even further?
With MIRO CANVAS, you can simply press start and step away. The entire library preparation workflow – including incubation steps, enzymatic fragmentation, PCR, and bead-based clean-up – are handled end-to-end, with reagent savings of up to 75 percent.
- Read our applications notes on how MIRO CANVAS can be used to automate NGS library prep protocols.
Check out the MIRO CANVAS
Scale-up solution for high throughput
VIAFLO 96 or VIAFLO 384 handheld electronic pipettes
Need to process more samples? Process full 96 or 384 well plates in parallel. Use the VIAFLO 96 or VIAFLO 384 to add reagents, perform bead clean-ups or mix at speed, maintaining uniform processing across every well.
Check out the VIAFLO 96 or VIAFLO 384
Add-on tools
SWITCH hybrid pipette with HMW DNA shearing capability*
HMW DNA shearing for long-read sequencing typically requires dedicated instruments.
Shear high molecular weight DNA to 20-23 kb fragments in under 5 minutes using a handheld pipette. The SWITCH performs in-tip mechanical shearing through automated aspiration cycles, delivering reproducible fragment sizes validated for long-read sequencing workflows. Use the same pipette for your daily pipetting tasks.
Read our application note on how to perform in-tip shearing of HMW DNA with the SWITCH.
Check out the SWITCH for HMW DNA shearing
*The SWITCH for HMW DNA shearing is available in Austria, Belgium, Canada, Denmark, France, Germany, Ireland, the Netherlands, Sweden, Switzerland, the United Kingdom and the United States.
MAG module + MAGFLO™ NGS magnetic beads*
Size selection and clean-up are sensitive steps in library preparation.
ASSIST PLUS or VIAFLO 96 or VIAFLO 384 automate bead-based size selection and clean-up using MAG module and MAGFLO NGS beads, supporting reliable bead capture and consistent fragment size distributions.
- Read our application note with ready-to-use scripts on how the ASSIST PLUS can be used to automate NGS size selection workflows.
Check out the MAG module and MAGFLO™ NGS magnetic beads*
* MAGFLO NGS magnetic beads are only available in Austria, Belgium, Canada, Denmark, France, Germany, Ireland, the Netherlands, Sweden, Switzerland, the United Kingdom and the United States.
Go further
I.DOT LT non-contact liquid dispenser**
Want to save reagent? Miniaturize dispensing with tip-free accuracy.
Dispense ultra-low-volume master mixes, adapters or enzymes, then keep the workflow seamless with VIAFLO 96 or 384 for bead-based clean-up and size selection.
Check out the I.DOT LT
** The I.DOT LT is only available in Austria, Belgium, Denmark, France, Germany, Ireland, the Netherlands, Sweden, Switzerland and the United Kingdom.