Stage 3: Sequencing ready
The challenge
Small volume differences matter in normalization and pooling.
Repetitive manual pipetting across many libraries can lead to inconsistent dilutions and variable pooling volumes.
INTEGRA automates these final steps, helping laboratories to ensure consistent library representation and move efficiently to sequencing.
Simple set-up for low to medium throughput
D-ONE single channel pipette module + ASSIST PLUS pipetting robot
Dock the D-ONE module onto the ASSIST PLUS to automate the pipetting steps for normalization and pooling. Define volumes and transfers once in intuitive software, then execute them hands-free from your worklist.
- Read our application note with ready-to-use scripts about how the ASSIST PLUS can be used to automate DNA normalization workflows.
Check out the ASSIST PLUS and D-ONE
Scale-up solution for high throughput
I.DOT LT non-contact liquid dispenser**
Want to save reagent? Miniaturize the normalization step.
Use the I.DOT LT for normalization by adding different volumes of diluent to every sample well - tip-free, precise, and efficient with every drop.
Once normalization is complete, hand your plates to the ASSIST PLUS with D-ONE to complete pooling.
Check out the I.DOT LT
** The I.DOT LT is only available in Austria, Belgium, Denmark, France, Germany, Ireland, the Netherlands, Sweden, Switzerland and the United Kingdom.
High quality, sequencing-ready libraries. Your workflow is complete.