The complete guide to ELISA

The enzyme-linked immunosorbent assay (ELISA) is considered the gold standard of immunoassays available today. It is a popular plate-based method for detecting and quantifying peptides, proteins, antibodies or hormones, and is used in a wide range of both research and applied settings, including clinical applications, such as the detection of viral infections.

We have compiled this detailed eBook – consisting of in-depth educational articles, relevant app notes and customer testimonials – to help you understand how ELISAs work, the materials required to perform them, and their limitations in comparison to other immunoassays, such as western blot. We also highlight what you should consider in order to run ELISAs successfully, and demonstrate how our solutions can help you to enhance the throughput of your lab and become an ELISA expert.

What's inside your free ELISA eBook

Chapter 1: What you need to know about ELISAs

In this chapter, we will cover the different types of ELISAs, analysis of ELISA data, and the different reagents and equipment required to perform these tests in the lab. We will also explain the differences and similarities between ELISAs and western blotting, and their respective pros and cons, to allow you to make an informed decision on which technique to use for your specific application.

1. An introduction to the different types of ELISA tests
2. ELISA vs. western blot: a comparison of two common immunoassays

Chapter 2: INTEGRA Biosciences' ELISA solutions

ELISAs are the mainstay for a wide range of studies but, unfortunately, generally involve multiple time-consuming and tedious processing steps that require consistent pipetting – from well-to-well and plate-to-plate – to ensure success. On top of this, the repetitive nature of this work can also frequently result in user fatigue and handling mistakes. Fortunately, with the right tools, you can quickly and easily ramp up your throughput, reduce errors, increase consistency and improve the reproducibility of your results. In chapter 2, we will demonstrate how INTEGRA’s range of liquid handling solutions can help you to save time and elevate your ELISA workflows for more successful experiments.

Chapter 3: Application notes

Our pipetting instruments are used across a broad spectrum of life sciences applications, and we endeavor to share knowledge and experience of using our products with the wider scientific community. To this end, we have compiled an extensive database over the years, which contains a wide range of thorough and useful application notes. In chapter 3 are some of the most relevant app notes for ELISA protocols and workflows.

1. Performing an ELISA with the ASSIST PLUS pipetting robot
2. Performing an ELISA with the VIAFLO 96 or 384 handheld electronic pipette

Chapter 4: Customer testimonials and product stories

Our range of innovative liquid handling products has helped many laboratories to achieve success with their ELISA projects, improving lab throughput and meeting their exciting research goals. But don’t just take our word for it! In chapter 4 is a small selection of real-life experiences from a few of our valued customers, along with a key product story, which illustrate why INTEGRA Biosciences' pipetting solutions and labware are the best choice for your ELISA workflow.

1. The Department of Biomedicine at the University of Basel shares its experience with the VOYAGER pipette
2. The University of North Texas shares its experience with the VIAFLO 96 pipette
3. Making COVID-19 ELISA testing safer and easier with the VACUSIP portable aspiration system

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