Everything you need to know about NGS

Next generation sequencing (NGS) has revolutionized genomics, empowering scientists with a powerful tool to sequence millions of DNA fragments at once. This transformative technology has increased the speed, scalability and throughput of sequencing, accelerating genomic research to broaden our understanding of genetic variations and gene expression. 

At INTEGRA Biosciences, we recognize the pivotal role that NGS has played in shaping the future of genomics research, so we have developed a comprehensive eBook to help scientists navigate this technique. This eBook guides the reader through different sequencing methods, exploring the advantages and disadvantages of each, and provides a series of educational articles, application notes and testimonials to show how INTEGRA Biosciences’ solutions can help to streamline NGS workflows.

What's inside your free NGS eBook

Chapter 1: What you need to know about NGS

DNA sequencing has progressed enormously since the inception of Sanger sequencing in the 1970s, and there are now various methods on the market, each with their advantages and limitations depending on your desired application. NGS techniques were developed to provide faster, more efficient and cost-effective sequencing methods, outperforming traditional approaches. Before embarking on your NGS endeavor, it is important to understand the different techniques and how they can be applied to your workflow. In this chapter, we will cover the history of the different DNA sequencing methods, and delve into the difference between Sanger sequencing and NGS.

1. DNA sequencing methods
2. Sanger sequencing vs NGS

Chapter 2: What INTEGRA can offer

Sample and library preparation for NGS involves a number of complex, multi-step processes that are labor-intensive, tedious and error-prone when performed manually. Fortunately, automating these steps and working with 96 and 384 channel pipettes can help to greatly enhance the throughput and efficiency of NGS workflows, while improving the reproducibility of results. In this chapter, we will explain how our diverse range of automated library prep and liquid handling instruments offers solutions for everyone to seamlessly improve their workflows, regardless of throughput or automation requirements.

Chapter 3: Application notes

At INTEGRA, we are always keen to share our knowledge and experience to help drive scientific advancements. In this chapter, we’ve supplied a selection of application notes that explain the best practices for our NGS library prep and pipetting solutions, and explain how they can help to streamline your workflows.

1. Enabling the Illumina DNA PCR-Free Library Prep kit on the MIRO CANVAS® NGS prep system
2. Automating PacBio® SMRTbell® whole genome sequencing library prep on MIRO CANVAS®
3. Automating the Oxford Nanopore Ligation Sequencing Kit on MIRO CANVAS®
4. Implementing the Twist Human Core Exome Kit on MIRO CANVAS®
5. PCR clean-up protocol with Agencourt AMPure XP magnetic beads
6. Beckman Coulter AMPure XP beads protocol for PCR purification
7. Automated size selection with MAGFLO NGS, the cost-effective alternative to AMPure XP
8. Automated DNA normalization for NGS library prep
9. Affordable, high throughput bulk RNA barcoding and sequencing plate preparation with the VIAFLO 384

Chapter 4: Customer testimonials

We are extremely proud of how INTEGRA liquid handling solutions are being used across the world to support scientific findings, from developing high throughput sequencing methods to solving the canine epigenome. In this chapter, we share a selection of testimonials from our customers, as they explain how these cost-effective instruments have helped to streamline and scale their research.

1. The Payne Laboratory at University College London’s Cancer Institute shares its experience with the ASSIST PLUS pipetting robot
2. Microsynth shares its experience with the MINI 96 and VIAFLO 96 electronic pipettes
3. Alithea Genomics shares its experience with the VIAFLO 96 and VIAFLO 384 electronic pipettes

4. The College of Veterinary Medicine at Seoul National University shares its experience with the VACUSAFE and VACUSIP aspiration systems

    

Download the eBook