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Increase speed and efficiency of loading agarose gels using the VOYAGER pipette
Complete your gel loading in the blink of an eye using a VOYAGER adjustable tip spacing pipette
Gel electrophoresis is a standard laboratory procedure that is indispensable for nucleic acid visualization and purification. Samples are loaded onto a rigid polysaccharide gel, then subjected to an electric field. This induces migration towards the anode due to the negative charge of the nucleic acids, separating the individual components by size and/or conformation. Typically, samples from 96 well plates or PCR strips are loaded onto agarose gels using a single channel pipette. Multichannel pipettes with fixed tip spacing cannot be used, because the spacing of the gel wells is usually different to the 9 mm spacing of well plates and PCR strips. The VOYAGER electronic adjustable tip spacing pipette is the ideal solution. It offers one-handed operation and automatic adjustment of tip spacing to enable straightforward aspiration and dispensing of samples from well plates or PCR strips into the pockets of an agarose gel. Gel loading is much faster than with a traditional single channel pipette, because multiple samples can be transferred simultaneously.
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Table of contents
Gel electrophoresis is a standard laboratory procedure that is indispensable for nucleic acid visualization and purification. Samples are loaded onto a rigid polysaccharide gel, then subjected to an electric field. This induces migration towards the anode due to the negative charge of the nucleic acids, separating the individual components by size and/or conformation. Typically, samples from 96 well plates or PCR strips are loaded onto agarose gels using a single channel pipette. Multichannel pipettes with fixed tip spacing cannot be used, because the spacing of the gel wells is usually different to the 9 mm spacing of well plates and PCR strips. The VOYAGER electronic adjustable tip spacing pipette is the ideal solution. It offers one-handed operation and automatic adjustment of tip spacing to enable straightforward aspiration and dispensing of samples from well plates or PCR strips into the pockets of an agarose gel. Gel loading is much faster than with a traditional single channel pipette, because multiple samples can be transferred simultaneously.
Key benefits
- The VOYAGER enables the simultaneous transfer of multiple samples in various labware formats, increasing speed, efficiency and throughput.
- VOYAGER pipettes feature a unique motorized tip spacing function, enabling one-handed adjustment of the spacing with a simple push of a button. It offers advantages over manual tip spacing adjustments, for example, leaving the other hand free to handle labware.
- The increased throughput of a multichannel pipette results in much faster work and fewer transcription errors, because the user does not need to go back and forth from the sample source to the gel very often.
- The VOYAGER enables much more precise and reproducible pipetting into the gel, as 8 or 12 channels can be pipetted simultaneously, at the same speed and height.
- The VOYAGER helps prevent repetitive strain injuries (RSIs) and pipetting errors by reducing transfer steps.
- The VOYAGER forms a perfect system in combination with GRIPTIPS®, which never leak or fall off.
Overview: How to accelerate gel loading with the VOYAGER
Experimental set-up
In this protocol, a VOYAGER 8 channel 12.5 μl adjustable tip spacing pipette with Low Retention, Sterile, Filter GRIPTIPS is used to load eight DNA samples from a 96 well plate into an agarose gel in one go. Tip spacing adjustment is needed prior to loading the samples onto the gel.
- Step 1: Tip spacing adjustment
- Step 2: Gel loading
Step-by-step procedure
1. Tip spacing adjustment
Different tip spacing set-ups for the 96 well plate and the gel
Before starting, the user needs to adjust the tip spacing in the Main Menu under Tip Spacing. The tip spacing of the 96 well plate and the PCR strips is 9 mm, and the gel comb of the 16 well gel is 4.5 mm. Choose 'Position 1' and set the tip spacing to 9 mm according to the 96 well plate, then choose 'Position 2' and set the tip spacing to 4.5 mm according to the agarose gel wells. After saving, select 'Pipet' mode in the Main Menu.
Tip:
- If required, it is easy to set up a different tip spacing. VOYAGER pipettes allow tip spacing from 4.5 to 33 mm.
2. Gel loading
Sample transfer from sample plate to the agarose gel
Set the required volume and transfer the first eight DNA samples from the 96 well plate into the agarose gel. Pressing the left or right arrow on the Touch Wheel will automatically adjust the tip spacing (Figure 1).
Tips:
- For better results, pipette the samples into the agarose gel slowly to allow them to run down to the bottom of the pockets and avoid introducing air bubbles. Setting the dispensing speed at 4 or lower gives optimal results.
- Low Retention GRIPTIPS demonstrate excellent results when pipetting the viscous sample-DNA dye mixture.
Repeat the transfer step and load the second set of samples into the empty wells of the agarose gel (Figure 2). The gel is now ready for electrophoresis.
Conclusion
- Gel loading with the VOYAGER multichannel electronic pipette is precise, reproducible and eight times faster compared to single channel pipettes, saving time for other experiments and eliminating transcription errors.
- Tip spacing can be changed to fit 96 well plate and the gel pockets with just the push of a button.
- The best pipetting results are achieved when using Low Retention GRIPTIPS for viscous liquids.
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Instruments and accessories
VOYAGER, 8 Channel, 12.5 µl
VOYAGER pipettes allow the tip spacing to expand anywhere between 4.5 mm and 33 mm at the push of a button.
- Single handed operation leaves the other hand free to handle labware.
- On the fly access to up to 3 user-defined tip spacings.
- These user defined tip spacings are saved. No need to memorize spacings of different labware formats.
Part No. 4721
GRIPTIPS®, 12.5 µl, Sterile, Filter
GRIPTIPS® pipette tips perfectly match the multi-lobe tip fitting, snapping firmly on during loading. This guarantees a perfect seal on every tip, preventing them from loosening, leaking or completely falling off. All tips are precisely aligned horizontally, enabling accurate touch-offs, even when pipetting with 384 tips.
Part No. 4415
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DownloadIncrease speed and efficiency of loading agarose gels using the VOYAGER pipetteCustomer’s voice
The Voyager is an absolute time saver! I use it to load from eppie tubes to PCR plates, load electrophoresis gels from PCR plates, it was the only pipette that covered the correct spacings. It's very easy to use and customise your own program.