The ultimate guide to stress-free pipetting
How to become a pipetting expert
Take the stress out of pipetting and improve liquid handling performance. Learn how to choose the right pipette and pipette tips, apply best practices for accurate and precise results, and handle challenging liquids such as viscous or volatile solutions with confidence. Whether you're optimizing everyday lab work or looking to reduce repetitive strain, this comprehensive eBook will help you make pipetting more efficient, reliable and ergonomic.
Pipetting is a fundamental skill in any laboratory as liquid handling protocols form the backbone of many life sciences workflows. However, pipetting improperly can lead to inaccurate results, repetitive strain injuries, and wasted resources. This eBook aims to equip you with the knowledge and skills you need to turn pipetting from a tedious necessity into an efficient, accurate and enjoyable part of your lab work.
Our educational articles and practical tips and tricks will help you to choose the right pipette and pipette tip for your application while addressing crucial ergonomic considerations and pipetting best practices, with a focus on achieving high accuracy and precision even when handling challenging liquids. We also present valuable information covering essential maintenance and calibration routines to prolong your pipettes' lifespans and ensure consistent results. This eBook additionally contains in-depth application notes illustrating how INTEGRA Biosciences' solutions can support you in optimizing your liquid handling protocols. We're confident that this comprehensive guide will play a significant role in helping your facility to achieve stress-free pipetting once and for all.
What's inside your free pipetting eBook
Chapter 1: What you need to know about pipettes
There are numerous pipettes on the market today, all with their unique attributes, key uses, and pros and cons. In this chapter, we will cover some of the main differences between the various types of pipettes and tips, what to consider when choosing the right solutions for your application, and how to protect yourself against injuries caused by extensive manual pipetting.
- Everything you need to know about the different types of pipettes
- Are you using the right type of micropipette?
- Ergonomic pipettes and pipetting guidelines
- The different types of pipette tips (and when to use them)
Chapter 2: Pipetting best practices
Inconsistencies in liquid handling technique can lead to poor data and reproducibility, compromising the integrity of results. However, it can be difficult to consistently operate pipettes, whether they are manual or electronic, even with years of experience under your belt. On top of this, certain liquids prove especially problematic, requiring particular skills to handle successfully. This chapter explains different ways you can easily improve your technique for greater precision and accuracy.
- Proper pipetting: 10 tips on how to use a pipette
- Pipetting tips to increase accuracy and precision
- How to pipette viscous and volatile liquids
Chapter 3: Maintaining and calibrating your pipettes
You need to recalibrate and maintain even the best quality liquid handling products to keep them in top condition and providing reliable and reproducible results. In this section, we discuss how you can clean, decontaminate and store your pipettes correctly for optimal performance. We also cover the importance of regular calibrations for confidence in your data and explain how you can check the accuracy and precision of your pipette.
- How to clean, decontaminate and store pipettes
- Calibration check: How to calculate the accuracy and precision of a pipette
Chapter 4: INTEGRA Biosciences' pipetting solutions
Learn how our extensive range of liquid handling solutions can empower you to create faster and more efficient pipetting workflows with fewer errors.
Chapter 5: Application notes
At INTEGRA, we always endeavour to share our knowledge and experience with the wider community to support scientific advancements and discoveries. In this chapter, we've included a selection of key application notes demonstrating how some of our electronic pipettes, pipetting robots and reagent reservoirs are used in a broad spectrum of life sciences projects.
- Fast and efficient sample transfer from plate to plate with INTEGRA's electronic pipettes
- Fast and efficient automated sample transfer from tubes to plates with the ASSIST PLUS pipetting robot
- Automated reagent and sample aliquoting with the ASSIST PLUS pipetting robot
- Efficient 2-way sample pooling automated with the ASSIST PLUS
- Measuring dead volume in INTEGRA's multichannel reagent reservoirs