I.DOT LT
Non-contact, low volume dispensing for reduced assay costs
Key Benefits
-
Reduce assay costs
Dispense as little as 17.3 nl, running up to 10x as many samples at the same cost. Ideal for assay miniaturization to maximize throughput and budget efficiency.
-
Conserve reagents
With a dead volume as low as 1 µl per source well, precious reagents are used not wasted.
-
Have confidence in your results
A built-in optical sensor recognizes when source wells are depleted and verifies the total volume dispensed in real-time, ensuring accuracy, traceability and peace of mind.
-
Generate high quality data
Dispense low volumes into any SLAS-compatible well plate with maximum precision.
-
Minimize your plastic use
Non-contact dispensing to minimize pipette tips usage and plastic waste, saving costs.
Small droplets – big impact
The I.DOT LT reduces reagent costs and enables assay miniaturization through precise, low volume, non-contact dispensing. It dispenses volumes down to 17 nl, with exceptionally low dead volumes of just 1 µl per source well, maximizing performance without sacrificing precious reagents. Integrated real-time volume verification ensures accurate, reproducible dispensing, which is essential for applications such as NGS, (q)PCR and compound screening. Its fully enclosed design protects reagents and samples from environmental contaminants, preserving integrity throughout the dispensing process.
Whether working with enzymes, primers, master mixes or small molecules, the I.DOT LT simplifies complex protocols, minimizes variability and delivers high quality results – all in a compact, walk-up device that is accessible to any lab!
Applications
PCR and NGS workflow miniaturization
Experience high throughput, miniaturized, semi-automated NGS library preparation with minimal set-up time. The I.DOT LT precisely dispenses enzymes, PCR master mixes, oligonucleotides and buffers, streamlining NGS and PCR protocols at a fraction of the original costs, while eliminating human error.
Drug discovery
Carry out efficient and reproducible dose-response studies for drug development without manual pipetting. The I.DOT LT supports target screening and potency/inhibition testing with low volume, sub-microliter dispensing of assay reagents, proteins, small molecule compounds and DMSO.
Synthetic and cell biology
Streamline biochemical and cellular assays to advance your synthetic and cell biology investigations. The I.DOT dispenses RNAi and CRISPR reagents and dyes in seconds, rather than minutes, speeding up workflows for higher productivity and faster results.
Take a look at our latest application note.
Cost-efficient miniaturization of 16S metagenomic library preparation
How it works
The I.DOT LT precisely delivers liquids in the range of 17.3 nl to 30 µl with an impressive 0.1 nl resolution. It performs non-contact dispensing by delicately ejecting droplets into target microplates using advanced positive pressure modulation. The I.DOT LT is designed for easy and efficient low-volume reagent dispensing and workflow miniaturization, making it accessible to every laboratory and unlocking new levels of productivity.
Non-contact, pressure-based dispensing
A dispense channel is formed between the dispense head and the liquid source well. Finely modulated pressure pulses enable droplets ranging from 17.3 to 50 nanoliters to be ejected from a 100 µm orifice at the bottom of the liquid source well, offering total dispense volumes up to 30 µl. The high quality disposable source wells are an integral component of the I.DOT LT dispenser, ensuring precise and reliable performance with each droplet dispensed.
Tech Info
| Volume range | 17.3 nl to 30 µl |
| Operating conditions | 18-25 °C <80 % humidity |
| Compatible formats | Any ANSI/SLAS-compatible well plate with a maximum height of 55 mm and a density of up to 1,536 wells |
| Dispensing technology | Non-contact, positive pressure dispensing (integrated compressor) |
| Dimensions (W x H x D) | 35 x 35 x 37 cm (13.8 x 13.8 x 14.6") |
| Weight | 15 kg / 33 lbs |
| Power requirements | 100-240 VAC, 50-60 Hz, 50 W |
| External air pressure supply (optional) | 2±0.1 bar / 29±1.5 psi, filtered 20 μM, oil-free |
Parts and numbers
| Description | Part no. |
| I.DOT LT, non-contact low volume dispenser | 5500-01 |
| I.DOT LT source wells, disposable, made of polypropylene, 48 pack | 5510-01 |
