Magnetic beads for NGS size selection and PCR purification in 1ml, 50ml and 500ml bottles

MAGFLO™ NGS Magnetic beads for NGS size selection and PCR purification

Magnetic beads for NGS size selection and PCR purification

Produktstory

Reliable size selection for NGS library preparation

NGS library preparation requires precise fragment size selection for high quality sequencing results. MAGFLO NGS magnetic beads are designed for exactly that: reliable single- and double-sided size selection across DNA and RNA workflows, plus efficient PCR clean-up. They deliver the performance you expect, with practical advantages that simplify your workflow.

MAGFLO NGS beads – key benefits:

USPs for the MAGFLO NGS beads
Funktionsweise

How it works

MAGFLO NGS magnetic beads are used for size selection in NGS library preparation, ensuring consistent DNA or RNA fragment sizes.

MAGFLO NGS double side single side size selection figure
  • MAGFLO NGS magnetic beads are RNase free, making them suitable for both DNA and RNA workflows. 

  • Precise bead-to-sample ratios ensure easy target size selection, offering effective targeting of nucleic acid fragments in both single- and double-sided size selection protocols without needing to change bead ratios when using the gold standard beads.

Kundenstimmen

Kundenstimmen

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Affordable and reliable

"I’ve transitioned to using MAGFLO NGS beads and have had no issues. They perform just as well as competing products, even at very low nucleic acid concentrations — and at a significantly lower cost."

Ian Pichler

University of Zurich

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MAGFLO NGS Beads: Reliable and Efficient

"We tested the MAGFLO NGS magnetic beads with INTEGRA’s MAG Module and observed increased reproducibility and fewer outliers compared to the standard protocol. The beads demonstrated excellent magnetic responsiveness, enabling efficient separation and a streamlined workflow. This combination enhances..."

Chiara Galeaz

Prebiomics S.r.l.

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Works flawlessly

"The MAGFLO NGS beads work as flawlessly as beads from competitors but cost only a percentage of the corresponding product of other brands."

Robin Wuigk

Repertoire Immune Medicines (Switzerland) AG

Gerät in Aktion

Gerät in Aktion

Anwendungen

Anwendungen und Applikationen

NGS library clean-up

NGS size selection during library preparation

Magnetic beads are adept at selectively isolating DNA fragments according to their size, a critical step for both single- and double-size selection in NGS library preparation. Employing MAGFLO NGS beads with MAG or HEATMAG modules, combined with the precision offered by INTEGRA's pipetting platforms, for magnetic bead purification ensures high yields while minimizing bead loss.

Find out how MAGFLO NGS magnetic beads can streamline your NGS workflow.

ASSIST PLUS transfers MAGFLO magnetic beads to PCR plate on MAG
Single size selection

Left or right size selection of nucleic acid fragments

MAGFLO NGS magnetic beads feature a single magnetic bead-to-sample ratio in single size selection.

MAGFLO ngs single side size selection figure
Double size selection

Left and right size selection of nucleic acid fragments

MAGFLO NGS magnetic beads feature two specific ratios in double-sided size selection.

DNA amplicon sequencing

Library preparation for the Illumina MiSeq™ System

Amplicons of 16S rRNA and internal transcribed spacer (ITS) were obtained from a microbial community standard containing genomic DNA from 8 bacterial species (3 Gram-negative and 5 Gram-positive) and 2 yeasts. This study shows the comparable performance of INTEGRA and AMPure XP magnetic beads when used in size selection for NGS library preparation.

RNA-Seq

Comparative analysis of gene expression patterns in RNA sequencing

RNA-Seq is an indispensable tool in modern biology, using NGS to analyze the complexity of gene expression dynamics and molecular pathways across diverse experimental conditions. In this comprehensive analysis, we show the interchangeability of INTEGRA MAGFLO NGS and AMPure XP magnetic beads in nucleic acid clean-ups for RNA-Seq of human reference RNA samples.

Technische Daten

Technische Daten

Finden Sie hier eine kurze Übersicht der technischen Daten. Ausführlichere Informationen finden Sie im Download-Bereich dieser Seite.

Application

Size selection for NGS library prep and PCR purification

Starting material

Fragmented DNA, RNA, cDNA, PCR products

Elution volume

12 µl or above (standard 96 well PCR plate)

Fragment size

Supports double-sided size selection from 100-1000 bp, also compatible with long DNA fragments and gDNA for long-read sequencing

Typical recovery

≥85 % (1.8x single size)

Processing mode

Automated and manual

Throughput

1-384 samples

Downstream applications

Library preparation for NGS / Sanger sequencing protocols, PCR, mutation detection and genotyping, fragment analysis, microarrays, enzymatic reactions, cloning

Selling unit

1, 50 and 500 ml

Storage

Store at 2–8 °C for maximum shelf life until the expiration date. Stable at 15–25 °C (RT) for up to 18 months, until the expiration date.

Intended use

Research use only

RNase free

yes

Downloads Bestellinformationen

Bestellinformationen

Magnetic beads

MAGFLO NGS

DescriptionPart No.
MAGFLO NGS, magnetic beads for NGS size selection and PCR purification, 1 ml, tube7000
MAGFLO NGS, magnetic beads for NGS size selection and PCR purification, 50 ml, bottle7002
MAGFLO NGS, magnetic beads for NGS size selection and PCR purification, 500 ml, bottle7004
Magnetic separation modules

MAG and HEATMAG modules and accessories

DescriptionPart No.
MAG module for magnetic bead separation4900
HEATMAG module for heating and magnetic bead separation4901
Adapter for 1.5 ml tubes, 4 x 6 format (MAG/HEATMAG)4905
Adapter for 96 well PCR plate (MAG/HEATMAG)4906
Adapter and magnetic array for 384 well PCR plate (MAG)4908
Adapter and magnetic array for deep well PCR plate (MAG)4907
ASSIST PLUS and D-ONE
96 channel electronic pipettes

Please visit these webpages for the MINI 96 and the VIAFLO 96 and VIAFLO 384 electronic pipettes.

VIAFLO and VOYAGER electronic pipettes