Clean Blood & Tissue DNA Kit Magnetic bead-based genomic DNA extraction kit
Magnetic bead-based genomic DNA extraction kit
A better way to extract genomic DNA
Spin column workflows require centrifugation between steps, multiple tube transfers, and can be difficult to automate. The Clean Blood & Tissue DNA Kit uses magnetic beads instead of columns to isolate high purity genomic DNA (gDNA) from blood, buffy coat, tissue and cultured cells – without centrifugation. The kit is compatible with manual workflows and INTEGRA automation for hands-free processing.
How it works
The Clean Blood & Tissue DNA Kit follows a 4 step workflow from sample to purified gDNA.
- Lysis: The sample is treated with a lysis buffer to break open cells, release nucleic acids and denature proteins and other impurities. Heating optimizes sample lysis.
- Binding: Magnetic beads are added to the lysate, where gDNA selectively binds to the bead surface in the presence of a binding buffer.
- Washing: Several wash steps remove proteins, salts and other contaminants, ensuring high gDNA purity.
- Elution: The purified gDNA is released from the magnetic beads into an elution buffer, ready for downstream use. Heating enhances sample elution.
The Clean Blood & Tissue DNA Kit delivers high genomic DNA yields from blood with consistent purity, as indicated by A260/280 and A260/230 ratios. The resulting gDNA is suitable for downstream applications such as (q)PCR and NGS. Read the full application note.
Use Clean Blood & Tissue DNA Kit with:
- Blood (citrate, EDTA)
- Buffy coat
- Tissue samples (up to 10 mg)
- Cultured cells (up to 25 µg gDNA from 5×106 cells)
This versatility makes the Clean Blood & Tissue DNA Kit well suited to genomic studies, clinical research and other applications where high quality DNA is essential.
In head-to-head testing with whole blood, the Clean Blood & Tissue DNA Kit produced higher purity genomic DNA than a leading spin column method, with comparable DNA yields and significantly reduced hands-on time (~5 minutes automated vs. 40–50 minutes manual).
The Clean Blood & Tissue DNA Kit works with INTEGRA's HEATMAG module, a magnetic stand that captures magnetic beads during binding and washing steps in bead-based workflows.
The HEATMAG also offers integrated heating – up to 65 °C – to enhance lysis and elution, helping to improve DNA yields. It is compatible with standard microcentrifuge tubes and 96 well PCR plates.
Combine the Clean Blood & Tissue DNA Kit with the HEATMAG module and ASSIST PLUS pipetting robot for automated workflows:
The ASSIST PLUS pipetting robot:
- Ideal for variable sample numbers
- Offers hands-free processing
- Provides intuitive, user-friendly programming
- Optimized pipetting protocols to process up to 24 samples that can be easily modified to accommodate differing input and output volumes
Le produit en action
Utilisations et applications
- gDNA extraction
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Take advantage of magnetic bead-based technology
The Clean Blood & Tissue DNA Kit delivers an automated, centrifugation-free workflow to simplify nucleic acid extraction. This eliminates the multiple time-consuming and labor-intensive centrifugation or vacuum steps associated with traditional spin column protocols. The result is high quality gDNA with excellent purity for a range of input sample types, including blood, buffy coat, tissue and cultured cells.
![DNA extraction performed on the ASSIST PLUS equipped with a HEATMAG module.]()
Spécifications
Veuillez trouver ici un bref aperçu des spécifications du produit. Des informations plus détaillées sont disponibles dans la section téléchargement ci-dessous.
Application
Starting material
Recommended input volume
Throughput
Intended use
Elution volume
Typical recovery
Processing mode
Downstream application
Unit size
Storage
Note
Références articles
- Clean Blood & Tissue DNA Kit
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Magnetic bead-based extraction kits
Description Part No. Clean Blood & Tissue DNA Kit 1x 16 preps 7100 Clean Blood & Tissue DNA Kit 1x 96 preps 7101 Clean Blood & Tissue DNA Kit 1x 384 preps 7102 - Magnetic separation modules
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MAG and HEATMAG modules and accessories
Description Part No. MAG module for magnetic bead separation 4900 HEATMAG module for heating and magnetic bead separation 4901 Adapter for 1.5 ml tubes, 4 x 6 format (MAG/HEATMAG) 4905 Adapter and magnetic array for deep well PCR plate (MAG) 4907 - ASSIST PLUS and VOYAGER
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Please visit these webpages for the ASSIST PLUS pipetting robot and the VOYAGER adjustable tip spacing pipettes.
