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D-ONE Single Channel Pipetting Module

Single Channel Pipetting Module

For ASSIST PLUS

Product story

Endless applications on the ASSIST PLUS – automated normalization, hit picking and master mix prep in a snap

The D-ONE single channel pipetting module offers hands-free transfers from individual tubes or wells on the ASSIST PLUS pipetting robot. This makes it the perfect add-on for labs that want to go beyond the possibilities of multichannel pipetting to access unlimited applications. It is designed to offer easy automation of normalization, hit picking and master mix preparation, freeing up your time while eliminating transcription errors.

How it Works

The ASSIST PLUS with the D-ONE single channel pipetting module offers automated, hands-off and worry-free processing.

  • Access unlimited applications

    D-ONE Overview
    1. The D-ONE single channel pipetting module is available in two volume ranges: 0.5 to 300 µl and 5 to 1250 µl.
    2. Pipetting with a single channel offers maximum flexibility, with automatic liquid level detection.
    3. A special D-ONE tip deck can accommodate two tip racks, allow the ASSIST PLUS to switch between 12.5/300 µl or 125/1250 µl GripTips without manual intervention.
  • Longer walk-away times

    D-ONE Volume Range

    Each D-ONE single channel pipetting module is compatible with two different GripTip sizes, providing a broad volume range without needing to change the pipetting tool during a protocol. This makes it ideal for workflows including high and low volume pipetting steps, such as plate normalizations with highly variable concentrations. Automatic selection of the correct tip size depending on the volume ensure optimal pipetting results.

  • Liquid level detection

    D-ONE Liquid level detection

    Different liquid levels in each sample are no problem at all! The D-ONE single channel pipetting module offers automatic liquid level detection, overcoming the need to specify fill volumes for easier and faster program set-up.

    Liquid level detection is automatically performed by the D-ONE, ensuring aspiration at the right height for optimal pipetting results.

    The D-ONE pipetting module senses the liquid level in real time during aspiration, and is compatible with all INTEGRA GripTips used for benchtop pipetting devices, avoiding the need for special tips when using the D-ONE.

  • Normalization and hit picking made easy

    D-ONE Hit picking

    With the intuitive VIALAB pipette automation software, setting up programs is a walk in the park, even for complex tasks like normalization and hit picking. The software features dedicated programs for these tasks, allowing fast and easy set-up.

    For normalization, a dilution calculator helps determine the sample and diluent volumes required, and automatically selects the best tip size to ensure high precision pipetting results.

    For hit/cherry picking, worklist files can be imported for fast program set-up.

  • Setting up pipetting tasks

    D-ONE dilution calculator

    With the intuitive VIALAB pipette automation software, setting up programs is a walk in the park, even for complex tasks like normalization and hit picking. The software features dedicated programs for these tasks, allowing fast and easy set-up.

     

     

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See it Work

See it work

Apps

Uses and Applications

Overview Applications Stories

Applications

Normalization
Normalization

Calculates the correct volumes of diluent for each sample

Normalizing or equalizing sample concentrations involves dilution calculations based on varying volumes of diluent and sample for each well. Using the D-ONE single channel pipetting module on the ASSIST PLUS allows hands-off, worry-free processing of this error-prone task. Only the initial and target concentrations need to be entered in the VIALAB software, which calculates the correct volumes of diluent for each sample. Broad volume ranges, from 0.5 to 300 µl or 5 to 1250 µl, ensure very high or low concentration samples can be processed without manual tool changes.

Normalization with D-ONE
Normalizing or equalizing sample concentrations with D-ONE
Hit or cherry picking
Hit or cherry picking

Sample transfers from single wells to the target plate

During hit or cherry picking, individual samples from several source plates are transferred to a single target plate for further analysis. Using the D-ONE single channel pipetting module on the ASSIST PLUS, sample transfers from single wells to the target plate are possible. Importing worklists to VIALAB makes programming effortless and minimizes set-up time.

Hit picking with D-ONE for ASSIST PLUS
Hit picking with D-ONE
Master mix preparation
Master mix preparation

Mixing individual components

The combination of several reagents – reaction buffer, primers and enzymes – at different volumes is the first step for preparing all PCR reactions. Using the D-ONE single channel pipetting module on the ASSIST PLUS, mixing the individual components can be easily automated. The number of reactions can be easily changed, and the total volumes are automatically adjusted in VIALAB. Broad volume ranges, from 0.5 to 300 µl or 5 to 1250 µl, ensure very high or low concentration samples can be processed without manual tool changes.

PCR Master Mix preparation with D-ONE
PCR Master Mix preparation with D-ONE
Standard curve preparation
Standard curve preparation

Precise serial dilutions

Precise serial dilutions are needed to prepare standard curves for quantitative assays, such as ELISAs or qPCRs. Standards are typically provided in individual tubes, and need to be serially diluted to cover the full dynamic assay range. With the D-ONE pipetting module, precise serial dilutions can be performed in tubes or directly in the assay plate. The correct tip is automatically chosen according to the diluent or sample volume, ensuring high precision pipetting results.

Samples with different filling level
Samples with different filling level

Detection of the liquid level

The D-ONE single channel pipetting module allows automatic detection of the liquid level, making it the ideal tool for pipetting varying liquid volumes into sample tubes. The sample is aspirated at the optimal height, where the tip is only just submerged in the liquid, to ensure high precision pipetting results. This eliminates tedious manual adjustment of liquid levels for faster program set-up.

Detection of sample level in tubes or other labware
Automatic detection of the liquid level
Tech Info

Tech info

Find here a quick overview of the product specifications. More detailed information can be found in the download-section below.

Compatible with
ASSIST PLUS
Number of channels:
1
Pipetting technology
Air displacement
Volume range
0.5-300 µl / 5-1250 µl
Battery
Rechargeable, Li-ion, 3.7 V, 1050 mAh; Typical charging time: 2.5 hours
Mains adapter
Input: 100-240 V, 50/60 Hz; Output: 5.7-6.4 V, 3 W
Downloads & More Parts and Numbers

Parts and numbers

Instrument
Instrument

D-ONE Pipetting Module

Description Part no.
D-ONE Single Channel Pipetting Module, 0.5-300 µl 4531
D-ONE Single Channel Pipetting Module, 5-1250 µl 4532
D-ONE All in one bundle
D-ONE 0.5 – 300 µl and D-ONE 5 – 1250 µl,
Tip Deck, Labware Pedestal, 2 x Communication Module
4539
D-ONE Single Channel Pipetting Module, 0.5-300 µl and 5-1250 µl
D-ONE Single Channel Pipetting Modules, 0.5-300 µl and 5-1250 µl
Accessories
Accessories

Tip deck and communication options for D-ONE

Description Part no.
Tip deck for D-ONE on ASSIST PLUS
(Compatible with 0.5-300 µl and 5-1250 µl D-ONE modules)
4535
Portrait labware pedestal 4551
Pipette Communication Module for D-ONE 4221
Charging/communication stand for 1 pipette, incl. mains adapter 4211

For more labware accessories, visit the ASSIST PLUS product page

GripTip pipette tips
GripTip pipette tips

Use the GripTip Selector Guide to find the correct pipette tips

D-ONE Single Channel Pipetting Module, 0.5-300 µl

Compatible with 12.5 and 300 µl GripTips for benchtop pipetting systems

D-ONE Single Channel Pipetting Module, 5-1250 µl

Compatible with 125 and 1250 µl GripTips for benchtop pipetting systems