D-ONE Single channel pipetting module

Normalizing or equalizing sample concentrations involves dilution calculations based on varying volumes of diluent and sample for each well. Using the D-ONE single channel pipetting module on the ASSIST PLUS allows hands-off, worry-free processing of this error-prone task. Only the initial and target concentrations need to be entered in the VIALAB software, which calculates the correct volumes of diluent for each sample. Broad volume ranges, from 0.5 to 300 µl or 5 to 1250 µl, ensure very high or low concentration samples can be processed without manual tool changes.

During hit or cherry picking, individual samples from several source plates are transferred to a single target plate for further analysis. Using the D-ONE single channel pipetting module on the ASSIST PLUS, sample transfers from single wells to the target plate are possible. Importing worklists to VIALAB makes programming effortless and minimizes set-up time.

The combination of several reagents – reaction buffer, primers and enzymes – at different volumes is the first step for preparing all PCR reactions. Using the D-ONE single channel pipetting module on the ASSIST PLUS, mixing the individual components can be easily automated. The number of reactions can be easily changed, and the total volumes are automatically adjusted in VIALAB. Broad volume ranges, from 0.5 to 300 µl or 5 to 1250 µl, ensure very high or low concentration samples can be processed without manual tool changes.

Quality control for NGS library preparation can be tedious and error prone if performed manually. Using the D-ONE single channel pipetting module on the ASSIST PLUS pipetting robot enables automated, worry-free normalization and pooling of your NGS libraries before sequencing. With broad volume ranges from 0.5 to 300 µl or 5 to 1250 µl, the D-ONE is well suited to the various concentrations used during library preparation. This all-in-one pipetting option avoids manual tool changes and minimizes human errors in the final steps of your NGS library preparation workflow.

Find out about all the potential applications of the D-ONE in NGS workflows here.

Precise serial dilutions are needed to prepare standard curves for quantitative assays, such as ELISAs or qPCRs. Standards are typically provided in individual tubes, and need to be serially diluted to cover the full dynamic assay range. With the D-ONE pipetting module, precise serial dilutions can be performed in tubes or directly in the assay plate. The correct tip is automatically chosen according to the diluent or sample volume, ensuring high precision pipetting results.

The D-ONE single channel pipetting module allows automatic detection of the liquid level, making it the ideal tool for pipetting varying liquid volumes into sample tubes. The sample is aspirated at the optimal height, where the tip is only just submerged in the liquid, to ensure high precision pipetting results. This eliminates tedious manual adjustment of liquid levels for faster program set-up.