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VIAFLO 96/384 Hand-held Electronic 96 And 384 Channel Pipette

Hand-held Electronic 96 And 384 Channel Pipette

Product story

Increase your pipetting throughput and reproducibility without investing in big automation

Previously, high throughput microplate pipetting was only possible by investing in fully automated liquid handling robots. These robots are not only expensive, they are also complex and difficult to program, requiring specially trained users.

That’s where VIAFLO 96/384 came into play. With this 96 and 384 channel pipette, INTEGRA meets the need for an affordable, compact and easy to use pipette that provides increased productivity. The unique operating concept makes VIAFLO 96/384 as easy to use as any traditional handheld pipette.

How it Works

How it works

VIAFLO 96 and VIAFLO 384 enable transfers of 96 and 384 samples in a single step, thus increasing pipetting throughput and reproducibility significantly. At a fraction of the cost, they often surpass throughput capabilities of even expensive robots.

  • Intuitive handheld operation

    VIAFLO 96/384 is a handheld 96- or 384-channel pipette designed to fit on any lab bench. It features a unique operating concept making it as easy to use as a traditional handheld pipette. Hold the control unit and push to move the pipetting head to the desired pipetting position. This unique servo assisted steering mechanism allows fast, precise and stress free multichannel pipetting without the need for position programming.

  • Quick and comprehensible menu navigatIon

    All INTEGRA electronic pipettes have a common user interface making it easy to switch between different systems without a second thought.

    A full color screen and proven touch wheel interface offer quick and comprehensible menu navigation. Simply by moving the finger over the touch wheel, menus and settings can be accessed and changed.

    VIAFLO 96 and VIAFLO 384 can be connected to a computer via USB port. With the free to use VIALINK PIPETTE MANAGEMENT SOFTWARE, user defined custom programs can be created on the computer and then be transferred onto VIAFLO 96/384.

  • Always work in the optimal volume range

    Users can simply change the pipetting head of their VIAFLO 96 and VIAFLO 384 to guarantee optimal matching of the available volume range to the application performed. Changing a pipetting head of VIAFLO 96 and VIAFLO 384 is easy and takes less than one minute.

    Each channel of a pipetting head is independent, having its own precise piston and sealing to exclude any cross-channel influences for error-free pipetting.

  • Space Saving Footprint

    With its compact design, this benchtop system requires little space on a lab bench and fits into most laminar flow hoods to allow work under sterile conditions. Up to three stage positions accommodate tip racks, microplates, reagent reservoirs or other SBS size labware.

    Up to 8 microplates can be stacked to make maximum use of the stage positions.

  • GripTip Pipette Tips Which Never Leak or Fall off

    Loosing a tip during an assay preparation can ruin hours of work. Also a reliable sealing between tip and fitting to avoid dripping tips is of key importance for accurate pipetting.

    INTEGRA has redesigned the connection between pipette tip and tip fitting to eliminate these issues. The unique GRIPTIP PIPETTE TIPS are comfortably loaded and ejected without any physical effort due to a power assisted mechanism activated by a push button. The loading procedure also guarantees that GripTips are not touched by the user and remain sterile.

Plate Holder Options

VIAFLO 96 and VIAFLO 384 feature two deck positions (A and B) to accommodate different plate holders.

Deck positions of VIAFLO 96/384

The plate holders can be removed and replaced by another option any time to adapt the instrument to various application requirements.

Standard plate holder
Plate holder with slide function

For 96-channel pipetting. The standard plate holder (6205) is used for simple 96-well transfers. The plate holder with slide function (6210) enables a grid offset to access 384-well plates with a 96-channel head.

Standard Plate holder / Plate holder with slide function

Spring loaded plate holder with slide function - 384-well plate offset

For 96- and 384-channel pipetting.
Available for position A (6215) and B (6220), these plate holders feature a spring load mechanism to secure microplates in place. Recommended for applications that require an exact plate positioning. Both holders also feature the slide function to access 384-well plates with a 96-channel head.

Spring Loaded Plate Holder A/B with slide function

Adjustable spring loaded plate holders with slide function – 384-well plate offset

For 96- and 384-channel pipetting.
Available for positions A (6223) and B (6224) these plate holders feature a slide function to access 384-well plates with a 96-channel head. A spring load mechanism secures the microplate in place. Both holders are adjustable in y direction to allow an optimal alignment of the pipetting head with the target plate. Recommended for 384-well plates with small wells, such as round well plates.

Adjustable spring loaded plate holder A/B with slide function

Adjustable spring loaded plate holders with slide function – 1536-well plate offset

For 96- and 384-channel pipetting.
Available for positions A (6225) and B (6226) these plate holders feature a slide function to pipet into 1536-well plates with a 384-channel pipetting head. A spring load mechanism secures the microplate in place. Both holders are adjustable in y direction to allow an optimal alignment of the pipetting head with the target 1536-well plate.

Adjustable spring loaded plate holde A/B with slide function

Three position stage

For 96- and 384-channel pipetting.
The stage provides three plate holder positions. Move the stage to the left and right to work with all three positions. It features the spring mechanism and slide function on all three positions The three position stage occupies both positions A and B and cannot be combined with another plate holder.

Three Position Stage

Row dilution plate holder

In addition to serial dilutions in columns, this plate holder enables serial dilutions in  rows. It works for both 96- and 384-well plates.

Row Dilution Plate Holder
Description Slide function
384 well offset
Slide function
1536 well offset
Spring loaded Adjustable Part No.
Standard plate holder - - - - 6205
Plate holder with slide function yes - - - 6210
Spring Loaded Plate Holder A/B with slide function yes - yes - 6215/6220
Adjustable spring loaded plate A/B with slide function yes - yes yes 6223/6224
Adjustable spring loaded plate A/B with slide function - yes yes yes 6225/6226
Three Position Stage yes - yes - 6230
Row Dilution Plate Holder yes - - (-)* 6221

 *plate holder slides back to front

Customer Voices See it Work

See it work

Apps

Applications

Reservoir to plate transfers

VIAFLO 96/384 fills microplates within seconds by transferring up to 384 samples simultaneously. It reduces pipetting errors and increases pipetting reproducibility as all samples are transferred the same way.

Time to fill five 96-well microplates [seconds]

The “Repeat dispense” function is an extremely effective mode to fill multiple plates with the same liquid: Aspirate once and dispense multiple aliquots of the same or different volume. Plates can be stacked during the process to minimize plate handling.

Typical applications of reservoir to plate transfers are:

  • Compound additions
  • Medium exchange
  • Cell seeding
  • Addition of buffers and reagents (e.g. ELISA handling)
Repeat dispense with VIAFLO 96
Plate to plate transfers

Click for more information

  • Plate duplication
  • Supernatant transfers
  • Plate reformatting 96 <-> 384 well

An exact aspiration height can be defined to precisely remove supernatant. The ability to adjust the pipetting speed helps to gently aspirate without disturbing cells at the bottom of a well.

Plate to plate transfer using a VIAFLO 384
Transfer within plates

Click for more information

Performing serial dilutions, mixing or resuspending contents of entire plates can be effortlessly accomplished without repeatedly pressing a plunger. The number of mixing cycles and the mixing speed can be exactly defined.

Serial dilutions using a VIAFLO 96
Nucleotide and protein extraction

Video demonstration

1536-well pipetting

Video explains how the special 1536-well plate holder enables easy and fast 1536-well pipetting with VIAFLO 384

High throughput screening

Molecular Partners from Zurich (CH) integrated a VIAFLO 96 in their protein expression workflow. The pipette is used for various steps in their IMAC protein purification protocol.

Integrating an INTEGRA VIAFLO 96 multichannel pipette into their high throughput expression facility has enabled Molecular Partners (Zurich, Switzerland) to streamline the discovery and development of a novel class of targeted protein therapeutics termed DARPins.

DARPins are a next generation of protein therapeutics combining the advantages of antibodies and small molecule drugs. High target specificity and potency, small size, cost-effective production and a whole range of possible multi-specific format choices gives them the potential to surpass existing antibody drugs and revolutionize protein therapeutics.

Andreas Lehmann, an Expert Technical Assistant working in Molecular Partners 96-well high throughput expression facility commented:

“Our lab is extremely satisfied with our decision to purchase and incorporate INTEGRA’s VIAFLO 96 into our development protocols. The main application we are using the system for currently is protein purification using IMAC (Immobilized Metal Affinity Chromatography). The VIAFLO 96 is used for various steps in the process starting from the preparation of bacterial cultures right through to the actual purification. Overall our protocol involves 63 full plate liquid transfers; such a workload would not be feasible with a traditional handheld multichannel pipette. We have found that the VIAFLO 96 is easy to integrate into our standard operating protocols because parameters can be defined: pipetting mode, volume, pipetting speed and pipetting height. We particularly like the repeat dispense function on the VIAFLO 96 as it saves the lab a lot of time and improves the overall reproducibility of tests as all samples processed at the same time in the same way".

Mr. Lehmann added:

"Being responsible for training of new staff, I also appreciate that the VIAFLO 96 is user friendly and intuitive to use because very little training is required".

Efficient Library Screening

PepsiCo Global R&D from White Plains NY (USA) uses a VIAFLO 384 to screen natural product libraries to find sugar substitutes. The 384-channel pipette is used to transfer compound plates to multiple cell plates.

Improving Efficiency of Replication & Screening

Using a VIAFLO 96 handheld multichannel pipette, the Institute of Genetics and Development of Rennes (IGDR) in France has been able to improve the efficiency with which they replicate yeast strain collections and perform genetic screens in 96 well plates.

The Institute of Genetics and Development of Rennes (www.igdr.univ-rennes1.fr) is one of the major biological research institutes in France. The objective of the IGDR is to develop research of excellence in areas including Genetics, Cell Biology and Developmental Biology. Employing 20 teams of scientists – current research interests at IGDR include investigations into cell polarity, cell cycle, signalisation, membrane trafficking, RNA stability, splicing, epigenetics, genetics and transcription. IGDR also study human pathologies like cancers, and genetics and orphan diseases.

Ewa Blaszczak, a PhD researcher at IGDR commented:

“We purchased the liquid handler from INTEGRA Biosciences nearly three years ago and are very pleased with it. We use it to replicate collections and yeast strains and to perform genetic screens in 96 well plates. Thanks to the speed and efficiency of the VIAFLO 96 we can, for instance, replicate a whole collection of 5784 yeast strains in less than one week".

She added:

"Our VIAFLO 96 has proven to be a great liquid handling device not only because of the speed and efficiency but also for its practicality. Set up is simple and operation intuitive. You can set-up a repeat mixing and dispensing application as well as change the aspirated liquid volume at any time. Moreover, its compact size allows you to fit it easily into any almost any laboratory space. I would strongly recommend the VIAFLO 96 for any type of 96 or 384- well plate applications”.

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Tech Info

Tech info

Find here a quick overview of the product specifications. More detailed information can be found in the download-section below.

Dimensions (W x D x H)
42 cm x 30 cm x 54 cm
Power Requirements
100–240 VAC, 50/60 Hz
Weight of VIAFLO 96 incl. pipetting head
25.7 kg
Weight of VIAFLO 384 incl. pipetting head
27.7 kg
Pipetting channels of VIAFLO 96
96
Pipetting channels of VIAFLO 384
384, 96
Compatible plate formats
96 and 384 wells, shallow and deep well
Plate positions
up to 3
Downloads Parts and Numbers

Parts and numbers

Instruments

VIAFLO 96 and VIAFLO 384 Handheld Pipette

Description Part No.
VIAFLO 96, 96-channel handheld pipette (base unit only) 6001
VIAFLO 384, 96- and 384-channel handheld pipette (base unit only) 6031
VIAFLO 96/384 base units
Plate Holders

Plate Holders for 96-, 384- and 1536-well plate

Description Part No.
Standard plate holder for 96 well plate 6205
Plate holder for 96 and 384 well plates, with slide function (384 offset) 6210
Spring loaded plate holder A for 96 and 384 well plates, with slide function (384 offset) 6215
Spring loaded plate holder B for 96 and 384 well plates, with slide function (384 offset) 6220
Row dilution plate holder for 96 and 384 well plates 6221
Adjustable spring loaded plate holder A for 96 and 384 well plates, with slide function (384 offset) 6223
Adjustable spring loaded plate holder B for 96 and 384 well plates, with slide function (384 offset) 6224
Adjustable spring loaded plate holder A for 96, 384 and 1536 well plates, with slide function (1536 offset) 6225
Adjustable spring loaded plate holder B for 96, 384 and 1536 well plates, with slide function (1536 offset) 6226
Three position stage for 96 and 384 well plates (occupies both deck positions A and B) 6230
Pipetting Heads

96- and 384-Channel Pipetting Heads

96-Channel Pipetting Heads (for VIAFLO 96 and VIAFLO 384)

Volume Range Part No.
0.5 – 12.5 µl 6101
2 – 50 µl 6106
5 – 125 µl 6102
10 – 300 µl 6103
50 – 1250 µl 6104

Note: 50 µl pipetting heads (Part No. 6106) are only compatible with instruments featuring a 2nd generation control unit (Firmware 3.xx or higher)

96-channel pipetting heads

384-Channel Pipetting Heads (only for VIAFLO 386)

Volume Range Part No.
0.5 – 12.5 µl 6131
2 – 50 µl 6136
5 – 125 µl 6132

Note: 50 µl pipetting heads (Part No. 6106) are only compatible with instruments featuring a 2nd generation control unit (Firmware 3.xx or higher)

384-channel pipetting heads
GripTips

GripTips for VIAFLO 96 and VIAFLO 384

GripTips in racks to use with 96-channel pipetting heads
(300 and 1250 microliters)

The tip racks are reinforced to guarantee proper loading of the GripTips.

Tip Size Color Code Description Sterile,
Filter
Sterile Non-
Sterile
300 µl Green 5 racks of 96 tips 6435 6434 6433
1250 µl Blue 5 racks of 96 tips 6445 6444 6443
GripTips in racks to use with 96-channel pipetting heads

GREEN CHOICE Refills for 96-channel pipetting heads
(300 and 1250 microliters)

The GREEN CHOICE is a environmentally friendly packaging of GripTips. Each GREEN CHOICE contains five GripTip inserts of non-sterile GripTips to refill the current tip racks.

Tip Size Color Code Description Non-Sterile
300 µl Green 5 inserts of 96 tips 6432
1250 µl Blue 5 inserts of 96 tips 6442
GREEN CHOICE refills for 96-channel pipetting heads

XYZ-racks for use with 96- and 384-channel pipetting heads
(12,5 to 125 microliters) 

XYZ GRIPTIPS for 96- and 384-channel pipetting heads feature a reinforced tip rack with SBS footprint. Additionally, carbon filled anti-static XYZ racks prevent static build up and allow for easy GripTip attachment each and every time.

Tip Size Color Code Description Sterile, 
Filter
Sterile Non-
Sterile
12.5 µl Purple 5 XYZ racks of 384 tips 6455 6454 6453
12.5 µl LONG Purple 5 XYZ racks of 384 tips 6405 6404 6403
12.5 µl SHORT Purple 5 XYZ racks of 384 tips 6475 - -
50/125 µl Yellow 5 XYZ racks of 384 tips 6465 6424 6423
XYZ-racks for use with 96- and 384-channel pipetting heads
Reagent Reservoirs

Reagent reservoirs with ANSI/SBS standard footprint.

Reservoir Base

Description Part No.
ANSI/SBS standard footprint (10 bases per case) 6300
Reservoir base

Disposable Reagent Reservoirs

(incl. 2 Reservoir Bases)

Description Part No.
100 ml, 96 well, 30 reservoirs individually sealed, sterile 6311
100 ml, 96 well, 200 reservoirs per case, bulk pack, sterile 6312
300 ml, 96 well, 30 reservoirs individually sealed, sterile, WHITE 6321
300 ml, 96 well, 200 reservoirs per case, bulk pack, sterile, WHITE 6322
100 ml, 384 well, 30 reservoirs individually sealed, sterile 6331
100 ml, 384 well, 200 reservoirs per case, bulk pack, sterile 6332
Disposable reagent reservoirs

SBS Polypropylene Reservoirs

Type Description Part No.
300 ml, 96 well polypropylene reservoirs 25 reservoirs per case, pyramid bottom, non-sterile 6351
300 ml, 96 well polypropylene reservoirs 25 reservoirs per case, pyramid bottom, pre-sterilized 6352
12 column polypropylene reservoirs 25 reservoirs per case, pyramid bottom, non-sterile 6361
12 column polypropylene reservoirs 25 reservoirs per case, pyramid bottom, pre-sterilized 6362
8 row polypropylene reservoirs 25 reservoirs per case, pyramid bottom, non-sterile 6371
8 row polypropylene reservoirs 25 reservoirs per case, pyramid bottom, pre-sterilized 6372
SBS polypropylene reservoirs
Cooling Plates

PCR Cooling Plates

Description Part No.
96-well PCR cooling plate 6250
384-well PCR cooling plate 6255
96/384-well PCR cooling plates
Replacement O-Rings

Red O-Rings for Tip Fittings

Description Part No.
200/300 µl, replacement kit, 24/pack 100-00027-50
200/300 µl, replacement kit, 100/pack 125928
1000/1250 µl, replacement kit, 24/pack 100-00028-50
1000/1250 µl, replacement kit, 100/pack 125929